FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1966-lungUL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1966-lungUL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058074
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4400.060.9878961
GCGAAAG4950.054.201212
CACCGTT450.00963995948.3324857
GCTATAT3150.046.0483441
CGAAAGC5900.045.4717453
GCCTATA1859.640644E-1139.203321
TATATAC5300.038.306643
CTATATA4100.037.1405142
TATACTG4500.037.0566525
ACGTATC950.00472782630.5257787
GTTCGTA1000.006075137328.999489145
GTAAGAC5400.028.1979453
ACCGTGC1555.5013785E-528.0640248
TATTAGG1306.476702E-427.8893992
GTATTAG2156.1058927E-726.9864711
GCTATAC1658.411918E-526.3669093
CGTATCT1100.00967266326.3631738
GTATACG1958.730645E-626.0350271
CGGAGCG1958.756311E-626.025183145
GTATTGA1409.973971E-425.9021931
Produced by FastQC (version 0.11.5)