FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1966-lungMR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1966-lungMR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255970
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16460.13105408568676002No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13530.10772550299768308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1859.640644E-1139.2029461
TACGGGG1406.61179E-736.2497337
GCGAAAG4450.034.220042
TATACTG5700.033.0712475
AGCGAAA4700.032.4049871
CGAAAGC5700.026.713523
TAGTACC5250.026.239994
GCGTTAA1450.001226671324.9998159
TTACGTG3201.2387318E-924.9266532
GTATTAT15050.024.0948351
GAATAAC8250.022.8565041
TACGTGT3253.9872248E-822.3101923
GTAATAT4851.6370905E-1120.935181
ATATACG1750.003657713820.715784
GTATATA4952.1827873E-1120.5122491
GTACCTA6400.020.3904746
GTATAAA9200.019.7080021
GTATATC2959.5364685E-619.6679171
TGCGTTA1850.00504779919.594458
GCTACTA3401.5155565E-619.1910349
Produced by FastQC (version 0.11.5)