FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1966-lungLR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1966-lungLR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556527
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1150.063.042319
AGCGAAA2300.056.7686771
GCGAAAG2750.047.4707222
CGAAAGC3800.036.262363
TATTCCG800.00203512836.2525865
GTATTAG2008.889401E-932.641991
TAGAGTG2900.032.5023165
TACACTG3050.030.9038415
GAATAAC2252.7981514E-829.0151021
GTATATG2405.2363248E-827.2016561
TGTAGTA1907.1440154E-626.712435
TTAGAGT3005.838956E-1026.5876184
AGTGATC3057.0940587E-1026.1470558
CCTATGC1958.722262E-626.0345153
CTTAAGC2859.838914E-925.4472683
GTATTAA3301.7571438E-924.1792531
GAGTGAT3102.4134351E-823.3866627
TATTAAA5000.023.207912
TATAATA4403.6379788E-1223.0760482
GTATAAG2552.6913021E-622.7569431
Produced by FastQC (version 0.11.5)