FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1966-lungLL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1966-lungLL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756123
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG18780.10694011752024203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2650.041.0561831
GTATTAT20800.031.035581
CGACCCG1204.0594337E-430.208143145
AGCGAAA6350.027.413891
TTACGTG4400.026.3679752
CGCATAA1100.00967383226.363472145
TATACTG5650.025.6650165
TATTATT28350.023.2754342
TATAGAC4103.8198777E-1122.9909363
GTTATGT9150.022.9884681
TAACCTA4750.022.8958975
TAGTACC7550.022.0878414
TACGTGT5001.8189894E-1221.752963
ATATACG2003.1026703E-421.7517224
GTGATCG2003.1044625E-421.7498659
TATAACC6700.021.6447353
GCGAAAG8100.021.4850162
GTTGACA16800.021.1553381
TATAGGT3106.2447543E-721.0500534
ACATGGT17150.020.7153385
Produced by FastQC (version 0.11.5)