FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1915_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1915_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802373
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC29000.3614279144487663No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG17300.21561044551598818No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT16730.20850651754234004No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG14790.18432823636887083No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCAGACCATTGACAAGG13550.1688740772683029No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA13040.1625179311866177No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC12140.1513012028071732No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA12080.15055342091521026No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT11680.14556820830212383No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC11560.1440726445181979No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG10500.13086183109351884No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA10240.12762144289501265No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT9600.11964510271407437No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA9530.11877269050678425No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT9410.11727712672285832No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC9270.11553230230827807No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC9010.11229191410977188No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG8620.10743133181201261No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC8470.10556187708210521No Hit
GTGTTAACTAGCACTGATTCTGGCCCGTTAATCTCCCACATCATAGACGA8440.10518798613612372No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC8380.10444020424416076No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA8360.10419094361350645No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC8340.10394168298285213No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA8280.10319390109088916No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG8240.1026953798295805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG1150.081.9611365
GTCATAT1150.075.67531
GCAGGTA1350.069.827452
CGAAAGC1051.6370905E-1162.1541063
GCGAAAG954.5474735E-1061.0636752
AGTAGAA2750.060.65491
TAACTAT2650.060.2070771
GACAGTA2800.056.9639437
TATTCCT1700.051.1793565
TATACTA3450.046.2402532
AGGTACT2050.045.981064
TTTGGAC3700.045.0757263
CTAATAG4300.042.1561664
TTCTACT700.001055899541.4334874
CCCTTAT3900.040.9099351
AAATATA907.478633E-540.280055
TACTAAT4550.039.842372
AGAAACA4200.039.7070924
TCTATGT1651.3624231E-939.5526123
TTATACT4050.039.387394
Produced by FastQC (version 0.11.5)