FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1915_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1915_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759349
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTC553.2185795E-452.7270367
TACGGTA450.00963842448.3331159
GCGAAAG2450.038.474282
AGCGAAA2850.038.1677741
GTGATCG1409.992634E-425.892749
CAATTCG2301.102575E-625.2172769
CGAAAGC4101.8189894E-1224.7592453
TCGATTA2651.3754834E-724.622528145
ATTATAC2951.4248144E-824.5793953
GTATAAG2101.5528976E-524.169743
TATAGTA3803.619789E-1022.8976482
GTATTAG3055.3234E-721.3989831
GTCCTAT7950.020.9802741
TATACTG4501.3278623E-1020.9457285
ATAACAC4752.728484E-1019.844633
GTATAGA5900.019.666111
AGTAGGC5407.4578566E-1118.7986873
GTAAGAC2701.0776828E-418.7986873
TTATACT4654.487447E-918.7108234
TATACAG4354.2635293E-818.3344575
Produced by FastQC (version 0.11.5)