FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1915_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1915_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGAGACTGGAGCTCCCCCATATAGACATGGAACAGGAACAGTATACA14.545454545454546No Hit
AACCAAGTAACTGACCATCAGCCCAAAACAAAGAACAGATTCTATTCATC14.545454545454546No Hit
CTTAAACCAACACCACAAAACAAGAACGCAATCGAGCACCAAAAAACATC14.545454545454546No Hit
AACTTGTGCGAGTAACAGGATAAGAAGGGGGCAGTTAGGATTCAGCCCAA14.545454545454546No Hit
ATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAA14.545454545454546No Hit
CTGCTGCACACTGGTCCTGTCACCGTTCGTTAATAAACCACCATTTTGAA14.545454545454546No Hit
TTAATCCAACACTTGAAAGCATTTAATGAAAATTTCATCATCAAATAATA14.545454545454546No Hit
CTATAGTACTTATGTATTGGCTGGATGCAATCATAACAATAAGATGTGAA14.545454545454546No Hit
GGGGGAACGGCAGGCAGCGGGAGACGCAGGGTCCCTGCTGAGCAAGGAGC14.545454545454546No Hit
GATAGATACCCAGCAACTGAATAAAACCACTTTATACAATTTCTACTTCG14.545454545454546No Hit
GATGATGACACTGAAATGAAATAACTAAACAAAGAAAATTTGAGAGGAAG14.545454545454546No Hit
CCCTAGGACTCCCGGAACTCTGTGGCCACCCTAGGGTCTGGATAGGAGAC14.545454545454546No Hit
CCATTAGGGGATGTATGAAATTATGATGTTGACAAAAGTTTAATCATTGA14.545454545454546No Hit
ACGAGAAAGTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCT14.545454545454546No Hit
GTTAAAACATCTCTGTGAGACGCACGCCGTCATCAACCTCATATCACACG14.545454545454546No Hit
AGTCAAGCCCCCGGGGACGGCGAGGAGGAGGAAGACCATCGAGGTACTAA14.545454545454546No Hit
GGTTTTTATTCTAGCCCTGCTCTCTTCATAAAGGGTGAAGTCAGCTTTAG14.545454545454546No Hit
CTCCATCACCATGCAAAAATAGCCATGATAGTCAACCTTATCTTGCACGA14.545454545454546No Hit
AGAACGATCACCAACTAACATCATATGTAATGCCACAAGACAATTCATTA14.545454545454546No Hit
GTCAAGTGCCTTCACCACAGGAGAGAGGATGCGACAGGATCCCTGCCCTG14.545454545454546No Hit
CACGAGACCAGTGGGGGAAGATTAGAATTTAAGTTTGGATAGCTGCGTTT14.545454545454546No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)