FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1915-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1915-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386774
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAACATGAATGACATTCAGGTGGCTGATATACAGAATGCTAGCA5440.14065061250239158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGT353.434698E-582.85677
GATAAGC300.001933890772.508982
ATAAGCG350.003563100762.150563
AGCGAAA858.879051E-751.2026751
GCGAAAG951.9161726E-645.7951472
GAGATCG657.331175E-444.6151476
ATACTAT855.3193013E-542.6688961
ACCTGCG855.335564E-542.646837
GTAATAG700.001053180441.4497831
AGGTCGT750.001482299438.666466
ATAGGAC1157.1253E-637.8307763
CTAATAC2002.2191671E-1036.2544943
TAGGTCG800.00203512736.2498055
CTGCGCG800.00203512736.2498059
ACCTAAT2052.8921932E-1035.3839651
TCTAGTG2551.8189894E-1234.1218762
GATCCGT850.002739988534.1174626
TAATACA2603.6379788E-1233.461364
TACAGGT1551.4619927E-632.741764
GTATACT900.003625596432.222054
Produced by FastQC (version 0.11.5)