FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1915-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1915-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749655
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7540.10057959994931001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1102.4356227E-432.9544269
GCTATAT2601.10958354E-1030.6852441
TATACTG3100.030.4031125
CTAGTAC1306.481563E-427.8845123
AGACGGT2405.268157E-827.187399145
CTATATA2456.425762E-826.6361082
TATTATA2207.4733E-726.3670582
TATATAC2808.18909E-925.8927613
GTCTAAT3251.4770194E-924.5481951
TATAGAT2151.8630575E-523.6077142
GCTACTA1902.1925122E-422.8946519
GTATTAT5450.022.6236481
GTATAGG2653.7779464E-621.8867136
TAGTATA2404.3611333E-521.1457564
GAATAAC4151.0859367E-920.9721721
AGCGAAA2455.0962204E-520.7225041
ATATACC2455.111957E-520.714214
CATGTTG7800.020.4486435
GTAGCAT11800.020.2834661
TAGCATG9000.020.14152
Produced by FastQC (version 0.11.5)