FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1915-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1915-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538041
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1755.2750693E-1141.4321025
CTACGGT800.002034928236.253094
GTAGGAC4750.035.1082573
TAGGACT4350.033.3361784
TACCGCA1102.4347712E-432.9542929
CGAAAGC2950.031.9518763
GCGAAAG3000.031.428112
TTATACT1753.767851E-629.0024724
TGTAGGA5750.027.7492312
GGACTGT5850.027.264756
AGCGAAA3204.1836756E-1127.1999321
TCCCGTG1606.805473E-527.1974012
CGTGGAT1358.073812E-426.8541415
GTCCGCC1358.0781966E-426.8516459
GAATAAC2507.7821824E-826.1119331
GTATATA2259.0582034E-725.7895621
CCTTATA2601.1386692E-725.1052932
ATCCTAC2651.3677891E-724.63391
ACTGTCC5500.023.7270938
ATGTAGT2151.8624309E-523.6066634
Produced by FastQC (version 0.11.5)