FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1914_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1914_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982642
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCG900.00362737132.223734
GTATATG3800.026.719931
TATAGGT2451.9112504E-623.6745744
AGCGAAA3152.8543582E-823.0239771
CTATTAC2252.6442796E-522.5634981
GTATTAT12600.022.4483781
GTATAAC2603.1942945E-622.3155481
CGAAAGC3253.9855877E-822.309873
TAGTACC5550.022.2071139
GCGAAAG3355.4929842E-821.6439022
AGACGGT2404.362925E-521.145744145
GTATAAG3157.256913E-720.7215791
GCTATAT3208.4418025E-720.3978041
CTAGTAC1850.005043968619.5965063
GTATTAG3151.6783828E-518.4191821
GTATAGG3552.281624E-618.3867531
CCTATAT2851.6285821E-417.8132861
ATATATC5401.5097612E-917.4554063
TCAGGCG4608.0897735E-817.3368829
CCGACTA3804.3803702E-617.1718544
Produced by FastQC (version 0.11.5)