FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1914-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1914-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601838
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12870.2138449217231215No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10100.1678192470399011No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT9100.15120348000624753No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA8930.1483787996105264No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA8620.1432279118300938No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG6160.10235312492730601No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT6040.10035923288326759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC554.3841283E-879.109821
GTGATCG701.6952752E-551.785189
TATACTG2600.041.8299675
CTATGCG951.0274592E-438.163844
GCTACTA1752.4265319E-937.2853289
GCGTTAA800.002036074936.2496269
TCAGCGA1854.1763997E-935.269918
CTACGCA1251.2629027E-534.8054282
CAGTGCA4050.034.0176472
TACGCAC1301.6519274E-533.4667553
GTATTAT6900.032.5802461
TAGATAG1153.1592656E-431.5240335
GCCCTAT2003.248624E-729.002116
GTAATAT2054.026224E-728.2994441
TACTGCG1050.007703317327.6210565
GATATGT3204.1836756E-1127.1939981
TATTATT7750.027.133262
CTACATC3503.6379788E-1226.9327683
GTTATGT2705.5406417E-926.8582691
CGCACTG1907.1451977E-626.7124715
Produced by FastQC (version 0.11.5)