FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1914-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1914-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829498
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC39080.471128320984499No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG34170.4119358937574292No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG31400.3785422026334No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC26040.3139248075341954No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG19450.2344791669178226No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT16520.19915659832814545No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA15910.19180275299036284No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA13780.1661245717289252No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG13630.1643162491048803No Hit
CCCTTAGGTGACTCTCCAATTGGCCATGTCTCCGATCTGTTTTCAAAGAA13400.16154348774801144No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC12640.15238131978618394No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC12530.151055216528551No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT10840.13068144829764508No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG10700.12899368051520316No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT10340.1246537062174954No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA9970.12019317707818464No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC9860.11886707382055171No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC9790.11802318992933075No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT9580.1154915382556679No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA9480.11428598983963795No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG9300.11211600269078408No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA9240.11139267364116609No Hit
GGCCTGGACAGTGGTGAATAGCATCTGCAACACAACAGGAGTCGATAAAC9110.10982546070032717No Hit
GTGGTGAATAGCATCTGCAACACAACAGGAGTCGATAAACCCAAATTTCT8930.10765547355147331No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC8910.10741436386826732No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA8690.10476215735300146No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA8690.10476215735300146No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA8670.10452104766979547No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA8420.10150717662972063No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT8420.10150717662972063No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA8410.10138662178811764No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG8360.10078384758010267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG100.007090734144.9994145
TAATCCG100.007090734144.99944
GCAGGTA7250.093.016432
GGTACTG7250.089.9996265
CAGGTAC9200.073.296583
AGGTACT10000.068.874714
AGTAGAA6400.063.456361
GTACTGA10600.062.924276
CGCAATT350.00356634862.142597145
ACGACAT350.00356634862.1425978
GACAGTA9150.057.841297
ACTGATC13200.053.2762958
TTTGGAC9950.053.1971473
AGCAGGT12550.052.5853541
CTGATCC12350.052.2467469
TATAGCG2050.045.9837382
TTTTTGG11500.045.40481
AACACTG9900.045.4038545
CGAAAGC2950.044.2451062
TTGGACA13650.043.5529334
Produced by FastQC (version 0.11.5)