FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1914-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1914-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796377
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC15180.19061323970933364No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC9080.11401635155209153No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT8520.10698450608191848No Hit
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT8050.10108277863373753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGC300.001932277172.531381
ACTACGC1001.3876586E-436.2656861
TAACGTC950.00472688530.5261027
TATACTG3400.029.8527345
GTAATAT2202.253546E-829.6719261
ACGTCCG1255.153117E-428.99989
CTAATAC2054.0310078E-728.297823
TCAGCGA1658.4168554E-526.3634538
TATACTA1658.4168554E-526.3634535
CGAAAGC4200.025.8975543
TATACCT2559.481664E-825.5880575
ATACCTA1751.2554711E-424.856976
AGCGAAA4101.8189894E-1224.766811
ATATTAA7350.024.6705361
TATTAAA7350.024.6658882
TAGGACT4550.023.9039344
CTATACT1851.8287389E-423.5163064
GCGAAAG4403.6379788E-1223.0738182
CTACGCA1600.002171678722.6617852
ATATAAG1952.606453E-422.3173471
Produced by FastQC (version 0.11.5)