FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1914-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1914-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720516
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG451.23256E-680.555295
AGATCGT1056.903065E-848.3331686
AAGATCG1451.9266736E-839.9998665
TATAGCG1303.6925303E-739.043752
GCGAAAG3150.036.8303872
TATTACG800.002036271636.2498784
CTATATA2750.034.277372
AGCGAAA3250.033.473041
ATCGTGC1352.1414253E-532.2221158
CCGACTA900.00362762232.2221154
CGAAAGC3450.031.5216333
GTATTAT4450.030.9656961
GTATTAG1504.386726E-529.0099681
CCTATAT1504.386726E-529.0099681
GCGGTAT1753.7727877E-628.9999056
CGGTATC1753.7727877E-628.9999057
TCGCCAT1255.152529E-428.9999145
CCCGATA1306.4760447E-427.8883932
GTATAAC1050.0076941327.6285421
TATATAC3750.027.0665783
Produced by FastQC (version 0.11.5)