FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1914-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1914-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875648
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG850.002742043534.1170279
CGACGAC2054.0388295E-728.29217145
TATACTG3800.026.7115695
CGAAAGC3555.456968E-1226.5503313
GCGAAAG3952.3646862E-1123.8630522
TATACAT5750.021.435625
GTATTAT11600.021.2581121
GTCTAAC2053.6564982E-421.2276131
ATACGAG1800.00430567920.1396753
GCACCGT3259.823161E-720.0777076
CCTATGC2256.8675814E-419.3329838
GTTGACA6550.018.8239771
GTACTAG1950.00682484218.596841
AGCGAAA4755.822585E-918.3227831
GTACAGT4404.8439688E-818.131921
TAGTACT3252.201725E-517.846854
GGGTACT2851.6329452E-417.8066947
GTCCTAT8250.017.5824661
TATAGAG2500.001392843217.4006795
GTACTTG2550.001590364817.0594886
Produced by FastQC (version 0.11.5)