FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1913_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1913_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382654
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAACATGAATGACATTCAGGTGGCTGATATACAGAATGCTAGCA5400.1411196537864494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGAC300.001933961572.508163
GCAGTCG350.003565076362.1417358
GTAATAG753.7521022E-758.006531
ACCTAAT1051.1041266E-955.244321
CTGCGCG701.6941818E-551.784789
ATTAGGT858.90328E-751.182243
GCGAAAG752.5459101E-548.3387761
AGCGAAA803.7278234E-545.3176041
TAATAGA1252.7106762E-740.6045724
ACCTGCG951.0278108E-438.1572077
TCCGTGG951.0278108E-438.1572078
CTAATAC2151.2732926E-1137.0972023
GAATAAC850.002738366434.1214941
TGTAGCC850.002740121734.117035145
TAATACA2705.456968E-1232.2258534
TTCCGTG900.00362577532.221647
CATAATA1153.156393E-431.5252882
TATACTG2309.968062E-1031.5252885
GACTACC1902.068864E-730.5297556
TATATAG1453.4821445E-530.003385
Produced by FastQC (version 0.11.5)