FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1913_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1913_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741921
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7520.10135850043333455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1354.9692426E-737.5924389
AGCGAAA3100.035.089961
GCGAAAG3100.035.0828632
GTATACG1458.676361E-735.0092931
CGTAGGG3000.031.4186526
CGAAAGC3600.030.2102413
CGGCGTA1703.0088522E-629.8548263
TATACGT1804.6955065E-628.1962242
GTAATAC1606.822862E-527.1892193
ACGTAGG1606.822862E-527.1892195
TACGTAG1606.822862E-527.1892194
TTAGACG1100.00966702626.3653054
TCAATAC1100.00966702626.3653053
ATACGTA1701.0304987E-425.5898513
CTCGGCG2051.2870301E-524.7626691
GGCGTAG2101.5536056E-524.1681944
TCGGCGT2101.5536056E-524.1681942
GTAATAT2552.6992893E-622.7511371
TATACTG3203.38041E-822.6576825
TGTAATA2252.6490554E-522.5569822
Produced by FastQC (version 0.11.5)