FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1913_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1913_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532145
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC701.6949494E-551.785488
CACCGTT901.322438E-648.3331157
GCGAAAG2353.45608E-1133.9392052
ACACCGT1301.6533842E-533.4613846
CCGTTCA1352.140397E-532.2220739
CGAAAGC2506.91216E-1131.9028573
TATTATA1153.158469E-431.5245572
TAGTATG2101.4359102E-831.074212
CGTTATA950.004723261630.5290452
AGCGAAA2651.382432E-1030.1055181
CCTTATA2202.2580934E-829.6617432
GTATTAC1753.75936E-629.0107731
TATACCG1000.006072071428.9998687
GTTCTAT4650.028.0749421
GTATTGA3650.027.818551
GTATAAG1050.007691307527.6293091
GTATAGA2903.929017E-1027.5102161
TATAGAA3204.1836756E-1127.189932
ATCCTAC2156.0957245E-726.9867651
TCTATAC4450.026.0697483
Produced by FastQC (version 0.11.5)