FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1913-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1913-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810389
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC29230.3606909768024985No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG17410.21483509771233322No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT16820.20755464351070907No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG14870.18349212538669699No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCAGACCATTGACAAGG13610.16794403675272No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA13110.16177416031066563No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA12250.1511619728303321No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC12240.151038575301491No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT11730.14474530133059554No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC11660.14388151862870796No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG10580.1305545855138705No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA10280.12685265964863787No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT9680.11944880791817264No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA9610.11858502521628503No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT9490.11710425487019198No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC9350.11537668946641674No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC9110.11241514877423064No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG8700.10735585009174606No Hit
GTGTTAACTAGCACTGATTCTGGCCCGTTAATCTCCCACATCATAGACGA8560.10562828468797085No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC8510.1050112970437654No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC8490.10476450198608322No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA8440.1041475143418778No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC8420.10390071928419561No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA8350.10303693658230802No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG8320.10266674399578475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA2950.083.6103441
GACTTAA353.4369994E-582.856575145
CGCAATT353.4369994E-582.856575145
ATTCCCG406.662651E-572.499504145
TAACTAT2650.068.437991
GTCATAT1200.066.498911
GGTACTG1154.0017767E-1156.7387435
TTAGGGG400.00605063754.37463145
GACAGTA2600.052.9804087
GCAGGTA1351.9099389E-1048.3419532
AACACTG3800.047.6970445
CTATTAG7550.046.1208531
TTTAAAC2550.045.498313
TATACTA4000.045.320582
ATTAGTA7600.044.8435253
AGAAACA5500.044.8178754
TATTAGT7650.044.5504262
TAGTAGA7700.044.2529455
AGAGGTC7900.044.0503359
CGAAAGC1002.7301467E-643.5077553
Produced by FastQC (version 0.11.5)