FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1913-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1913-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766338
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG554.059202E-665.9131852
CGCTATA651.0929998E-555.7726942
TAAATCG450.009638404548.333185
TAGCGGT1001.3911717E-436.2498864
GCGAAAG3150.034.525952
TTAGGCG900.003627762732.222124
TAGGCGG900.003627762732.222125
AGCGAAA3400.031.995631
ATAGCGG1153.1602645E-431.5236953
CCGCTAT1204.0494956E-430.2180941
GTATAAG1453.4816512E-530.0096971
GCGGTAT1255.152825E-428.9999086
GTATAGT2802.6193447E-1028.4913481
TAACGTA1050.00770746327.6189615
CGGTATC1358.081128E-426.8517677
CGAAAGC4250.025.5898253
GGTCGTT1450.00122620424.9999227
ACGGTGC1952.6140257E-422.3076238
AAGGTCG2003.1021883E-421.7499315
ATATTCA6850.021.1678164
Produced by FastQC (version 0.11.5)