FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1913-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1913-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890779
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG9350.10496430652271775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC3350.043.288143
GAACCGT855.3412426E-542.646766
AGCGAAA3650.041.7331241
GGTACTG4500.040.279765
GTACTGC1051.8536304E-434.5235676
TATACTA2404.3655746E-1133.23085
AGCGGTA1402.7454196E-531.0729565
GTACTGA5650.030.7962466
CCCGTAG950.00471551130.5415331
TAGCGGT1453.487463E-530.003164
ATAGCGG1453.487463E-530.003163
GCGAAAG4850.029.9034122
TACTGAT5900.029.491327
TATAGAC1753.7709124E-629.0030543
TATACTG5000.029.0014295
GATCCGG1000.00607431228.99986
AACCGTA1050.007708270627.6188557
GTACCGA2903.947207E-1027.499817
CTATACT2654.5511115E-927.3613724
TATAGCG1606.817207E-527.1934172
Produced by FastQC (version 0.11.5)