FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1912_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1912_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTAGGCCAGTGCCTTCCCCAGCCATGAGGAGACGCGAGAGGATCACT30.2540220152413209No Hit
GGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGATCC30.2540220152413209No Hit
GGATGGCACTCTGCTCACAATGCCATCTCTCGGGGTAGGGATGGCCTGCC20.1693480101608806No Hit
ATTCAGGATAGATCAATAGAAGATGCTCTTGTCTGAGAACTGAAACCCCA20.1693480101608806No Hit
CATCCTGGGTTCGTCCCGCCACGCCAAAATAAGCAGACACAGCCTCGCCC20.1693480101608806No Hit
GTGTTGGGCCGAGTGACTGTGTCCTGTTCTACGAAGCCTAAGCAAGTTTG20.1693480101608806No Hit
ATGCTATGCTTTCACAGAGGTTTTCAGCTTGGCGCGAGCCGCACATCGCT20.1693480101608806No Hit
ATCCAAAAGAAAAGGGTACATTTCCAGAAACATACAACCTACTAAGATTG20.1693480101608806No Hit
TCTAGAATGTCAAGAACTATGAAGGTTGGAGATACTGTCCTCCACATAAG20.1693480101608806No Hit
CACGTGGAGATAATCGACGATGCCGTCACCGTGGCTTTGAAGGACTGACA20.1693480101608806No Hit
TCCTTTTTCTGGGTGTACCTTATTGTTGACTCATTAACATTGACCTCACG20.1693480101608806No Hit
CGTTTAAGGACTGGCTTATTCCCGGGACTACTGTGACTGGCCTAACAAAG20.1693480101608806No Hit
GCCCAGCCAGGAGCACCCTCCGGCCTCCTTCCTCATCCGCAGTCTTCTCT20.1693480101608806No Hit
GCCTTATGGTGGTGTTTTAAAAATGGCCATAAGAGCAGTCCTGGATTCGA20.1693480101608806No Hit
GGGAGGCACTTAAGGGATTGGATATGTACCTCTTCCCTTTTTGGTCTCTC20.1693480101608806No Hit
CCCTTTCCCCTTTTATCTTTTCACACCCACTTTCTATTACACAAAAGAGG20.1693480101608806No Hit
CCTTTATGCCCGAACTTTTCGAAGTAGCTTGCTCAGCAGGCTTGGGTGGG20.1693480101608806No Hit
CCAGGACACTGAGGTCATGACCTGAGCCAAAGTTGGACACTTAAATGACC20.1693480101608806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTA100.006202039145.01
TTTTTTT400.007022043718.12565-69
Produced by FastQC (version 0.11.5)