FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1912-rep2-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1912-rep2-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119230
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11640.10400007147771234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG2100.041.4283269
TACGGGG1301.6550228E-533.461347
TTACGTG2351.2605597E-930.8550192
GTTACGT2701.70985E-1029.555351
GTGATCG2003.2550997E-728.999839
ACTTCGT1100.00967234426.3634828
CATAGTG3801.2732926E-1124.8024864
CTAATAC3551.546141E-1024.5090893
CATTCTA11600.022.5028842
CGAAAGC4850.022.4245533
AGCGAAA4659.094947E-1221.841491
ACGTGTT3355.500624E-821.6416654
CCGACTA2704.4458684E-621.4813564
TATACTG5450.021.2842775
TACGTGT3457.499693E-821.0162493
GTCATAG4201.2660166E-920.7169422
GTATTAT14850.020.5177631
GTATATC2857.061244E-620.3634951
CTACTGA15350.020.3093286
GTAGCAT19450.019.7680321
Produced by FastQC (version 0.11.5)