FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1912-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1912-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757728
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA553.2143548E-452.7410471
AAGGTCG1553.468449E-837.419259
GTCCTAT5400.030.88771
TCCTATG4650.028.0681462
GTACTGC1958.751018E-626.025576
TAATACG1409.99251E-425.8927864
GCGAAAG4850.025.4156552
TATACTG4950.024.8989225
GTGATCG1801.5198396E-424.16669
TAGTACC1500.001493963424.16669
AGCGAAA5150.023.9382921
GATTAAC1851.8268578E-423.5196591
TAATACT3402.5011104E-923.4558184
ATACTAT1902.1925286E-422.8946726
TTATACT4256.184564E-1122.1764094
GTATAGT2003.0966027E-421.7556841
CGTAATA1700.003089792821.3262862
GTCCATA2755.1961433E-621.096421
GTGTTAC5550.020.9063631
GTATACT1800.004306192520.1388344
Produced by FastQC (version 0.11.5)