FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1912-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1912-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70009
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGAGCATTG3630.518504763673242No Hit
CATCCATAAAATGGGCCTATGATATAACCTACCTCCTTCAGTGGGGGAGG3090.441371823622677No Hit
GTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGAG3010.42994472139296375No Hit
CTGTATGGCTGTGGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTC2860.4085189047122513No Hit
GTCTTATATCAGAGGAGGTGAAGCCACACTGGCATCTTATAAGTATATGG2610.3728092102443971No Hit
GCTGTGGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTCATCCATA2410.34424145467011386No Hit
AAACAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAA2260.3228156379894014No Hit
GTAGAAACAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGA2150.3071033724235455No Hit
AGTAGAAACAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGG2120.30281820908740303No Hit
GTATATGGGGACCCACTTTCTTCCTGGTGACCACTAATTTTCCTTTGCTG1950.27853561684926226No Hit
GATATAACCTACCTCCTTCAGTGGGGGAGGACCACATTTTAAGAATGTGG1820.2599665757259781No Hit
ATATAACCTACCTCCTTCAGTGGGGGAGGACCACATTTTAAGAATGTGGT1800.2571098001685498No Hit
CCTATTACCTCATCCATAAAATGGGCCTATGATATAACCTACCTCCTTCA1790.2556814123898356No Hit
GTATGGCTGTGGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTCAT1710.24425431016012228No Hit
CTATTACCTCATCCATAAAATGGGCCTATGATATAACCTACCTCCTTCAG1700.2428259223814081No Hit
AAGCAGGACAGGGAGAAGCAGGTTTCCTATTGACCAGGGAGCCCGATGTG1490.21282977902841063No Hit
GGTTTTGCCTTTGCCATTTATTTTCTGTATGGCTGTGGGCTGTTTGCTTA1490.21282977902841063No Hit
AGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTG1480.21140139124969648No Hit
GTATTGAATTGTTCTCTAAAATAGCTATACCAGGGACTCCTGAGTGGCTG1350.1928323501264123No Hit
GGATTAGAATTTTCAACTAGGATGAATGTTGAGCATTGTCACCACTCAGA1330.18997557456898398No Hit
AAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCCCCACTGAGCA1310.18711879901155568No Hit
GGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGA1300.1856904112328415No Hit
TTTATTTTCTGTATGGCTGTGGGCTGTTTGCTTAGCTTTTCTGAACCCCT1280.18283363567541316No Hit
TCCCTAGTCTGACCTCACTCTCTCGTTTCAAAAGTAACCAATGTCTTAGT1260.17997686011798483No Hit
ATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATCAGAGGAG1250.17854847233927065No Hit
GGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTCATCCATAAAATG1200.17140653344569984No Hit
GATGAATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATCAG1190.16997814566698566No Hit
TCTTATAAGTATATGGGGACCCACTTTCTTCCTGGTGACCACTAATTTTC1170.16712137010955735No Hit
CATTTTAAGAATGTGGTACATGAAAGTGCTGAAGCAAAATGCCATTAATA1140.16283620677341484No Hit
GTCAAATCCAGGTTTTGCCTTTGCCATTTATTTTCTGTATGGCTGTGGGC1140.16283620677341484No Hit
CTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTTGGTAGAGAATATG1140.16283620677341484No Hit
GGATGAATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATCA1090.15569426787984403No Hit
GTACATGAAAGTGCTGAAGCAAAATGCCATTAATAGTCCTGGTATTATTA1090.15569426787984403No Hit
GAAACAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGA1060.15140910454370152No Hit
GGACAGGGAGAAGCAGGTTTCCTATTGACCAGGGAGCCCGATGTGTGGCT1050.14998071676498734No Hit
AATCCAGGTTTTGCCTTTGCCATTTATTTTCTGTATGGCTGTGGGCTGTT1050.14998071676498734No Hit
CAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGT1000.14283877787141652No Hit
GCGAAAGCAGGGTCAAATCCAGGTTTTGCCTTTGCCATTTATTTTCTGTA960.13712522675655986No Hit
TGATATAACCTACCTCCTTCAGTGGGGGAGGACCACATTTTAAGAATGTG940.13426845119913156No Hit
AGCGAAAGCAGGGTCAAATCCAGGTTTTGCCTTTGCCATTTATTTTCTGT940.13426845119913156No Hit
CCCTTGGTTTTATCATCCTTGGAAATAGTCTCATGACAGCTAAGTGAAGA920.1314116756417032No Hit
GTAAATAGGTGAATATGTCCAAATAACCAAATCAGCCATAAAAGTAGTTC910.12998328786298904No Hit
GTATTTGCCTTTCATACTCCCTTGGTTTTATCATCCTTGGAAATAGTCTC910.12998328786298904No Hit
CGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGAGCAT890.1271265123055607No Hit
CTTATAAGTATATGGGGACCCACTTTCTTCCTGGTGACCACTAATTTTCC880.12569812452684656No Hit
TATCAGAGGAGGTGAAGCCACACTGGCATCTTATAAGTATATGGGGACCC880.12569812452684656No Hit
AAAGCAGGCTGTGAAAGGAGCAAGAGGCTAAGAAGCTGCTCCGCCCTCAA870.12426973674813238No Hit
GATTAGAATTTTCAACTAGGATGAATGTTGAGCATTGTCACCACTCAGAG820.11712779785456155No Hit
GTCACCACTCAGAGGGCATTTGTCTTATATCAGAGGAGGTGAAGCCACAC780.1114142467397049No Hit
GAATATATTCTGTTGAATTAACAGCGAGCAAATTTTAAACCACAGAACCA770.10998585896099074No Hit
TCCCAGTACCCTGGGATCATGACCTGAGCCAAAGGCAGAGCTTAACCAAC740.10570069562484824No Hit
GTAGATAAAAGTGCACACGAGGGATGAGAACATTAATTACATCCAAAGAA730.10427230784613407No Hit
GGGAGAAGCAGGTTTCCTATTGACCAGGGAGCCCGATGTGTGGCTTGATC720.1028439200674199No Hit
CTTATGAACCTGTATTTGCCTTTCATACTCCCTTGGTTTTATCATCCTTG710.10141553228870574No Hit
TTTCTGAACCCCTATTACCTCATCCATAAAATGGGCCTATGATATAACCT710.10141553228870574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG100.007076882145.01
ACTAAAC100.007076882145.07
GATAGGG203.8508902E-4108.752
AGCGAAA650.0100.384621
CCTAGTC301.5890386E-596.6666643
GAGCGAA301.5890386E-596.6666641
GGGTAAG259.3500596E-487.01
CCCTAGT406.620026E-572.52
GGCTTGC406.620026E-572.57
GGCTTAT406.620026E-572.51
TCTGACC451.1861472E-464.444448
TCCCTAG451.1861472E-464.444441
CTTATAG451.1861472E-464.444443
AGTGGCA350.00355266762.1428535
ATAGGTG606.739914E-660.4166685
GCGAAAG1102.5465852E-1159.318182
CTGACCT501.9972981E-458.09
TTATAGT501.9972981E-458.04
CAGGACA501.9972981E-458.04
TATAGTG501.9972981E-458.05
Produced by FastQC (version 0.11.5)