FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1912-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1912-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727074
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA8240.11333096768692044No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT8170.1123682046119102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTCG1402.728484E-1046.606775
GGTCGTT1557.403287E-1042.0964367
AGGTCGT1752.4283509E-937.2854166
GTCGTTT1951.70985E-1037.179198
TATACTG6750.035.4441575
TTAGGTA2103.783498E-1034.5259064
GTATTAT7050.029.830661
CTAGTAC1000.006071581529.0017593
TTATACT7150.028.3933324
GTAATAG1050.007704808427.6207243
GTTGACA4700.026.2304081
CCGTAGA1701.02904356E-425.5950721
GAACCGA1701.03096965E-425.588036
CCGACTC1751.2553332E-424.8569439
CCTTATA7400.024.4947322
GGATAGC2151.8649454E-523.604465
AGCGAAA3402.4938345E-923.4621491
ATACTGG7200.023.1595366
TAGGTAG3152.8625436E-823.0156885
GTAATAT2603.1947548E-622.3136521
Produced by FastQC (version 0.11.5)