FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1912-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1912-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367663
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT73742.0056410354047047No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG63111.7165175717980867No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT61001.6591280602073093No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG59241.6112581358472298No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT56881.547068919091668No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA53861.4649284807010767No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA48901.330022330231761No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG39941.0863208971259006No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC37021.0069003408012227No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA30820.8382676527145783No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA27350.7438877450273755No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC25520.6941139032211563No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG23010.6258448633667244No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC20280.5515920829672826No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG20130.5475122598684121No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC19570.5322809202992957No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG19120.5200414510026845No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA19000.5167775925235882No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA18570.5050820996401596No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT18200.49501853599627926No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG18200.49501853599627926No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG17670.480603161046937No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT16430.44687662342960804No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG16000.4351811305461795No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG16000.4351811305461795No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG15720.4275654607616214No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT15650.42566154331548184No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT15240.41451002684523597No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC14710.4000946518958938No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT14260.3878551825992825No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA13990.3805115010213157No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA13470.3663681142785649No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA13290.36147232655992034No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC13260.3606563619401463No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA13110.3565765388412758No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG12420.3378093525864718No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT12110.3293777181821396No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT11570.31469035502620607No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT11450.3114264965471097No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT11270.30653070882846517No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT11180.30408281496914297No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG11130.30272287393618613No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC11010.2994590154570898No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC11000.2991870272504984No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA10230.27824393534296354No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG10120.2752520650704585No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT9960.2709002537649967No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA9950.2706282655584054No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA9850.26790838349249174No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT9760.26546048963316954No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA9650.2624686193606645No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG9540.25947674908815954No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA9480.25784481984861135No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA8930.24288546848608644No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT8730.23744570435425918No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT8650.23526979870152828No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA8520.23173395201584057No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG8480.23064599918947515No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT8460.23010202277629244No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG8270.22493424685105656No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT7990.21731857706649838No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG7900.21487068320717614No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA7770.2113348365214884No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA7680.20888694266216618No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA7600.20671103700943527No Hit
GCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGC7490.2037191667369303No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT7460.2029032021171562No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATC7410.2015432610841994No Hit
GAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAAT7340.19963934363805988No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG7260.19746343798532898No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT7230.19664747336555488No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT7210.1961034969523721No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA7010.1906637328205449No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG6940.18875981537440537No Hit
ATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATA6880.1871278861348572No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG6880.1871278861348572No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG6850.1863119215150831No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA6840.18603993330849175No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA6830.18576794510190037No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG6810.18522396868871766No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTG6650.18087215738325585No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG6620.18005619276348178No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG6540.17788028711075088No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC6530.17760829890415952No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC6500.17679233428438543No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA6490.17652034607779407No Hit
ATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCA6430.17488841683824588No Hit
AGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGAT6240.16972064091301No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC6160.16754473526027913No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT6060.1648248531943655No Hit
TTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAA6010.16346491216140868No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT5980.1626489475416346No Hit
AACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAG5900.16047304188890368No Hit
GAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAA5890.16020105368231233No Hit
TAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTG5870.15965707726912962No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC5760.15666520699662462No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA5580.1517694192779801No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA5560.1512254428647974No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC5470.14877754900547513No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT5430.14768959617910968No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT5410.14714561976592694No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG5380.14632965514615287No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA5330.14496971411319606No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC5280.14360977308023926No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA5270.1433377848736479No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA5260.14306579666705652No Hit
GAACACATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAGAGACC5200.14143386742750835No Hit
ACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATT5190.141161879220917No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT5180.1408898910143256No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA5160.1403459146011429No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT5100.1387139853615947No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC5070.13789802074182064No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA5010.13626609150227245No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA4990.13572211508908974No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC4960.13490615046931564No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA4920.1338181976429502No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA4850.13191428019681067No Hit
GATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAAT4840.13164229199021932No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCC4840.13164229199021932No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT4820.13109831557703658No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA4820.13109831557703658No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG4800.13055433916385387No Hit
CCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACA4790.1302823509572625No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA4760.12946638633748841No Hit
TCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCA4720.12837843351112296No Hit
ATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCA4700.12783445709794022No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGA4640.12620252785839206No Hit
ATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGC4620.12565855144520932No Hit
AGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACT4590.12484258682543525No Hit
GTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTTAAGAA4580.12457059861884388No Hit
CAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCA4570.12429861041225253No Hit
TCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGA4570.12429861041225253No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA4490.12212270475952162No Hit
ACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC4480.12185071655293027No Hit
CTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTG4470.12157872834633891No Hit
AGACAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTC4430.12049077551997346No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA4340.11804288166065119No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG4330.11777089345405983No Hit
CTGCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCAT4260.11586697600792029No Hit
GAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGA4160.11314709394200667No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA4150.11287510573541532No Hit
CAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAG4120.11205914111564123No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA4090.11124317649586714No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGA4080.11097118828927578No Hit
TTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACA4040.10988323546291033No Hit
GTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT3970.1079793180167708No Hit
GAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATCAATAT3940.1071633533969967No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAG3910.10634738877722263No Hit
CAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTA3910.10634738877722263No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA3900.10607540057063125No Hit
TAAAAGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTGGAGA3880.10553142415744854No Hit
TTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAG3870.10525943595085718No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG3860.1049874477442658No Hit
AAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTT3740.10172358926516946No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC3720.10117961285198675No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA3720.10117961285198675No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC3710.10090762464539538No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAG100.0070863874145.018235
AGTAGAA11850.0113.8737261
GCAGGTA7400.0111.718422
AGCGAAA1700.0106.6890951
CAGGTAC7900.0104.647643
GGTACTG8250.0100.1944055
AGCAGGT8350.098.179511
TTTCGTT950.091.578145
GGTCAAA2850.089.094751
GTCAAAT2950.086.039472
ACTGATC10050.082.959348
GTACTGA10000.082.649156
CTGATCC10150.081.427739
TAGGACT451.2304099E-680.565684
GCGAAAG2350.077.147852
TACTGAT10800.076.526997
GTAGAAA17900.076.1651842
TAGAAAC17850.075.972263
CAAATAT3350.073.591354
GAGCGAA300.001929629872.5485841
Produced by FastQC (version 0.11.5)