FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1911_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1911_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595801
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12780.21450115055194605No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9980.16750559331051812No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT9060.1520641959311918No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA8860.14870737041394694No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA8550.14350429086221742No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG6130.10288670210355472No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT6010.10087260679320781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCG100.0070903813144.9984145
CCGACTA1351.024091E-842.9660954
GCGAAAG2450.041.4455071
CGAAAGC2750.036.914882
ACTACGC800.00203187536.2648161
GTATATC1001.3874345E-436.2648161
GCGTTAA800.00203522736.2526449
GTTGACA7200.032.2353941
GTATTAC1402.7371723E-531.0841311
ACATGGT7600.030.5285425
GTATTAT8500.029.8651451
CACCGAC2104.9739174E-727.623382
GCTACTA1855.807671E-627.4344339
CCACCGA2405.243237E-827.1986121
CTACGCA1100.00966111726.3677732
GACATGG9100.026.2931254
TATACTG2259.0913636E-725.7796575
TAGCATG12750.025.5922492
GCATGTT12750.025.5901014
CATGTTG11950.025.4830325
Produced by FastQC (version 0.11.5)