FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1911_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1911_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGG30.1664816870144284No Hit
ATAAACAGGTGGGTCAGTGCCAGGGAATTTCACAGCAGAGACCTAGGGTG20.11098779134295228No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT20.11098779134295228No Hit
GCAGATGCCTGTAGGCTTTGCCAGTTCTGTGGAATATGTGTGAGGCTTAG20.11098779134295228No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA20.11098779134295228No Hit
GTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTC20.11098779134295228No Hit
GTGCTGGGGATATAATAACGACCAAGGTCACTGCCCTCATAGAGCTTTTA20.11098779134295228No Hit
CCTCAGAGTCCACTTTTCCAGATCCTACCCTTTCTTCCAAGTCCAGCTGG20.11098779134295228No Hit
GGGCTCTGCTCAGCAGGGAGCCAGCTTCCTCCTCTGTCTCTGCCTGCCTC20.11098779134295228No Hit
CTTGAATCCCACCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACA20.11098779134295228No Hit
AGCTGGAAGAGCTGGTGCGTTCCTTCCTGCCGGAAGCGCCGTTGTGGGTC20.11098779134295228No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA20.11098779134295228No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA20.11098779134295228No Hit
CTCCCAGCTGGGTTCTCAAACCCCACCTCTTCCTGCTCTCATGTGCCCCC20.11098779134295228No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCCCCTCGA20.11098779134295228No Hit
GTAATTTGATTACGACGAATTTGGATATACAGAACTGACATGAGATTCCC20.11098779134295228No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT20.11098779134295228No Hit
ATATTGTTGTGAGCGCGCTGGAAATGAATGAGGGCGGTTTGTCACAGGTT20.11098779134295228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT400.00733804418.12525-29
Produced by FastQC (version 0.11.5)