FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1911_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1911_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGGACCATGACCTGAGCCAAAGGCAGACGCTTAACGGACTGAGCCA10.6172839506172839No Hit
CCTCCTCTAATTTCTTAACTTTCTCCAAGTTTATCCACTTCCTCATAATG10.6172839506172839No Hit
ACTCTGAGCATGCTACAAAGAAATGGAAGGTTTTTATAGGAAGAAGGGTG10.6172839506172839No Hit
ATGAATGACCCATGAATCCTGTCCTGAAGGGGCTCTCGGTTCAGCGTGGG10.6172839506172839No Hit
GACTGACAGGCCCTGAACTTCTCCCAGGGCATCCATTTTGGCTGGAGAGA10.6172839506172839No Hit
CATCCTTCACTGAACCCTCCCACCAAGCCAGCTCCTCCTGACGGGTGAGG10.6172839506172839No Hit
ATTCAAATCTTCTGTTGTCACGCTTGTTGTTGTGTCGTTTCTTATGTACA10.6172839506172839No Hit
GAATCAGGTTTAGGGGGCAAGAACCACCACGTATGGGAATGTGAGAGTAC10.6172839506172839No Hit
ACCTACGTTTGGGCCCGGCGGAAATCGGGGAAATGCGGCCCTGACGTAAG10.6172839506172839No Hit
CCAGAAGGGCATGCTACGCAGAAAGCCTCTGTCACAGGCTGGCCAGCTCC10.6172839506172839No Hit
GAATTTAAGTCTCTTGCTGGGGAGAGGTAAACACTGCTCAGTTATTTGTG10.6172839506172839No Hit
TTACACAGCAGGGCAATAATCTATTGAAATAAGCCCGAAAAGGGGGCAGC10.6172839506172839No Hit
GAGTGAGATGGGGAGGAATAAACCAAAATTCACACAAGTAAGTCAAGTTC10.6172839506172839No Hit
ATAATGAGGTCAGCTGGAGGTGGTCTTGAGTGTCAGGTAGAGCAGCAGAT10.6172839506172839No Hit
GAAGCGGTGGCACTGGGGCCCTAAGGGTTCTCTAAAGAGAGGCTTTCACA10.6172839506172839No Hit
CATAAAATGAGGTGGGGAGCGTTTCTTCCTCTTGTTTGTCTGGCAGAGTT10.6172839506172839No Hit
CATTATGTACTCAGTGGCCCTGCAATGAGACACTTCCGCTGTGAAGTAGT10.6172839506172839No Hit
TCTGAAGACCAAACTGGCTCTGGAAAGCTTGGCTTTAGTTACCTGTCTTC10.6172839506172839No Hit
CTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCCTCCTGAAAGAACG10.6172839506172839No Hit
TCTGAAAGATGAGGTGATGAGACTTGAGGATGTTTGAGTTCCTGGATCCA10.6172839506172839No Hit
GGCTTAGTACAGGAGGGAGGTAAGGAGTAGTGGGCTAGGGGACGTGTTGT10.6172839506172839No Hit
CCAGACTCCGTATACATGAGGCTGTCTTCTCCGCACTTGCGAGCCCGCCA10.6172839506172839No Hit
CTGTGGACACGCTAACAGAAACCAATGTTCCTGTGACACATGCCAAAGAA10.6172839506172839No Hit
AGTCTAATCATACAAATGCTGTGGATATTGGATCGACGGAATCGGAAGGT10.6172839506172839No Hit
CATCCAAAGTTCAAGGTTTCGCCTTCCATGCAATGTCCCAATTTTTATGT10.6172839506172839No Hit
CAGTGGGCTCCAAATCTTTCAGGAAGTAGTTGGGGGAGGAGAGGAGACGG10.6172839506172839No Hit
TCAGAAGGGAGTGTGAATACAAAGCATCTTCAGAAAGAAAGTGACACAGG10.6172839506172839No Hit
GACTCAAAAGAGCGGTTTTTACCCTGTTCAAGACGCCCAATACACAAATA10.6172839506172839No Hit
GTTCTCAGCGCTGTGGTGTAGGGGGAGGCCCTTTGATTCTGCCGGGCTGA10.6172839506172839No Hit
GGCCCTATCTCAGGTTTGAAGGTCCTATTCAAATATTCTGTTGTCACGAT10.6172839506172839No Hit
CTCCACAGAGCAGGGACTCCAACGTGGGGCTCGATCCCAGGACCCTGGGA10.6172839506172839No Hit
CCCTGGCACTGCCCCACCTGTTTATGGACAGCAAAGCTAGAGAAGGTGAA10.6172839506172839No Hit
GTAGTTGCAACGGCATCATATTCCATGCTCTTGGCCGGACCATGGGCTGG10.6172839506172839No Hit
AAACAGTACATTGCCATTCCACAATATCAGTAAATATGCATTTGGAACCT10.6172839506172839No Hit
CCCAACAAACTATAACGAGCAAACAAATTTGTAAATAAGAAACGCAACAA10.6172839506172839No Hit
GGTCTGTGATTGCTAAGAAGCTGTCAGGAAACCTTCTCAGGGGAGAAGAA10.6172839506172839No Hit
CTGTTGAGCCCATAGATCTCAAACTACTTGAAGCTCAACATTTGCCTTTT10.6172839506172839No Hit
GGGTGGGGGGGGGGGGTGCGCCCGCGCGGGGGCGGGGGGGGGGGGGGGGG10.6172839506172839No Hit
GTAGAAAAAGCTTGGGAACTCAAATGGTCCTGGCCGATTTATGTAAGACT10.6172839506172839No Hit
CTCTCATCCAAACTACCGCACGGATAGTTACGCGAATAATGAACCAGCAT10.6172839506172839No Hit
CACTTAGCACCCTCATTTTATTTAAATAAATCATGATAATAATTAGACCA10.6172839506172839No Hit
CACCTCGGATCCCCAGGGAGTCCGGAGCTTGCTTATTGCCGGACATGTGC10.6172839506172839No Hit
GTATCTCATTCTTCCCTGGTGTTATCCACTGACCAGTAAATTCCCCCACA10.6172839506172839No Hit
GAGCCAAAGGCATATGCCCAAAGACTGAGCCACTAAGAAACCACATACAC10.6172839506172839No Hit
TCCTTATGTGGCCTTCCCTCCATGTGTCTCTGTATCCTAATCTCCTCTTC10.6172839506172839No Hit
ATTATTAACTGAGAAAAAGCTAACAAGATAAAATCCCAGAAAACACCTAT10.6172839506172839No Hit
GCTCTCTCTAATTCCAAGCTGTTTCCCACCCCACCCTACCCCCTTTCCAT10.6172839506172839No Hit
GTCCCTCGGGTGGATGCTCTCCTCACTCTAGTTGCTGAGTTTTTTATGCT10.6172839506172839No Hit
TCCCAGGACCATGATATCATGCCCTGAGCTGAAGGCAGAAGCTTTAACCC10.6172839506172839No Hit
ATGCCAGGCCGAATTAAGTAGACACAGCCACAACGCTGCAGTAGCGTGTG10.6172839506172839No Hit
GTCAGAATGAGTCTCATCGACATTCTACCATACATCCCTCATATTCCTGC10.6172839506172839No Hit
GCTTTGCGACCGTTTCCCGGCCACAAGGCCAACGGCTCTTTCTCTTCTCC10.6172839506172839No Hit
CAGTATTTTCTCCTCCAAGACCAAGCTAGAGTCCTTTTGTATGTTTTGTA10.6172839506172839No Hit
CTCCGGTGCAACGCGAGACAGGAGGTCCAGCTCTCGGACTGCACACAACC10.6172839506172839No Hit
GTTCGATCTGAATACCTCTATGGTGTTGGCCAAAGCAGTTGCAGCCGGCT10.6172839506172839No Hit
GGCTTAGGGCGGGGGTGTGGGTGGTTCGTGGCTGCGGGGGTGGGTGGGGG10.6172839506172839No Hit
ATGAGAGTGAAGTTGCTCGTGCTGGGCTGCCTGGGGTCCTACCCCTGGTA10.6172839506172839No Hit
AGATCCCGTAATCCTCACAAGGATTACACATGACGGGGCCCCTGGGCATA10.6172839506172839No Hit
TCGGTCTTCTGACTGGAAACAGATCCTAGCTCTTCATAGGGAAATTACGT10.6172839506172839No Hit
GGCCAAGCCCTTGAGCGTCTACGGGTCGTCTCCAGCAAGTCCCAGAGCTC10.6172839506172839No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC10.6172839506172839No Hit
GGTTGGGGAAACACTAGAAGTAGCCGATCGGCAAGGTCTCGATCCTGATG10.6172839506172839No Hit
GACATTGAACTACACAATTACAAACGTTTAAATCAGTCTTCAGGATTCTT10.6172839506172839No Hit
GGCCCAGCACTAGCCAAGGGCTTGTCACTTACCAAGCATTTGGTGAATTT10.6172839506172839No Hit
TTGCATCCCTGAAATACATCTTACTGGATCATACCTCTTCCCGTATTGTA10.6172839506172839No Hit
GTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGAGAGGAATGGAGGCAGCC10.6172839506172839No Hit
ATCTGGGCTTGAAGGGTGGATTTCAAATCATTTTTAACAACACGGCACTT10.6172839506172839No Hit
GGTCACAGGGTGGTCCTTGGGAAGGGGTAGTGGGCACAGAGGGATTGGCT10.6172839506172839No Hit
GTATTAATACATTCGAACTTGTGCTTTGGCAGAGTAGCCTGTGTTAGAAT10.6172839506172839No Hit
GGTTATGACACCAGGGACCACCTGGAAAGGATCCCAACATCCCCCCGCCC10.6172839506172839No Hit
CATCTATAGTGTTGGTTGCTGAAGATCCAGAGGAGGTAAGCCTTGAAACA10.6172839506172839No Hit
AATTAGATAAGCCCTTGAAGGATCTCTAGTCGGAGGGGGTTACAGGCACC10.6172839506172839No Hit
ATCCAGTACAGAAGCCCTGGTTTCAGCGACCTGGGCATTGAACTCCAGCG10.6172839506172839No Hit
CATATAAACAAAGCGCTGGTTCTTTGAAAAGAACAAAGCCTACAGAGTCT10.6172839506172839No Hit
TGTGAAGACAGAATGAGTCAGGGAAATTTGCCAGTAATGTGGGTATAGCA10.6172839506172839No Hit
TCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAA10.6172839506172839No Hit
GAGCCAGTAGGTAAGACCAGACAATGGCAAGAACCAGTTTTAGGAGTAAA10.6172839506172839No Hit
GTCCTGCTCCGCCCCGCATTTTGAGACCTGAGGCGGGTGTTCTCGCTGTG10.6172839506172839No Hit
GCTAATCTCCAGTGACAGAGGCTGCCTTGACCTACGTGGCTTGTGCTGGA10.6172839506172839No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGC10.6172839506172839No Hit
GACGAGAAGAGACCCAAACCAGAAGAGAATGAAGACAGGTGTGGAGAACC10.6172839506172839No Hit
TGGGCGCAGCAGACCGGAGGCGGGAGGGGGGGAGGAGCATGGGCGGCAGG10.6172839506172839No Hit
ATTCTAGACCCATGCACTATTGAAGGCCTAGTCGATGGCACCCCTGCGTG10.6172839506172839No Hit
CTGGGAGAGCTTCTTAATGTTTTTCTGCCTCAATTTCCTTGTCTAGGGCA10.6172839506172839No Hit
GGGACTGTTCTAAACTAAAAGGAGGGGTCAGCCAATACAGTATGTAGGGA10.6172839506172839No Hit
GAAGTGGGAGGAGCCAGTAGAGGGATGACCTCATGGTCCAGGTGACACAC10.6172839506172839No Hit
GGGCTCTCCTTGCCTCTGCGTGGCCCTTCTCACAGGGTTTAAGTTTTGAA10.6172839506172839No Hit
CAACAATGATAACTAAGATAATCATCCAACCTAATAGCACAAGACCTTAC10.6172839506172839No Hit
TCCCTACCTGCCAATGAGGCTGCAACAGTGTCTTTAAAGTATTTTTGTTT10.6172839506172839No Hit
CTTTGTGGGGTTATATAGAGACCGGTGTGTTTGGCTTTCCAGCTCGATGT10.6172839506172839No Hit
CTTAAACTGCATGCTGCTACTTCCACTTTGCTCAGTCTGTCTTTCTCTCT10.6172839506172839No Hit
ATGCTGGGTGGGTAAATATTTATAAATGTTGCATCATTTTACTGCATTAA10.6172839506172839No Hit
CAGAGACCCCTACCGATGCCACAGAGGTGATACACAAATTCAAACTAGAA10.6172839506172839No Hit
GGTCTAGGGGAAAGTGATGGGCAGAGCCCAGAAGGCCCCTGGGCTCCTGG10.6172839506172839No Hit
GCAGAAGCACAGGTCGGGGGGGCAGTGATTTGGTAGCAGCGCTATCAAAT10.6172839506172839No Hit
GAATGCACACTGTGGGTGGGACCTGATCTAGAAATGCCAATCATGTCTCA10.6172839506172839No Hit
GAACAATAACAGATAAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCCG10.6172839506172839No Hit
CTCTAGGACCGTCGAGGCTGCTTCGAGGATTTTTGAGAGAAATTGGACGC10.6172839506172839No Hit
TATCTAGGGGGTGGAGGGTGGTTGGTTGTGCTGGTTGGTGTCATTGCTTG10.6172839506172839No Hit
AGGATGAACACAGGAGGGAGGTCACGTGGCCTCGGTGTCCAGGGGCCTCA10.6172839506172839No Hit
TCGTTAGGTCCAGTGTCGACCTCTATGGCAGCGAGGACTGAGTGCCCCCA10.6172839506172839No Hit
GAGGGAGAGAGGGCAGCAGGGGCAGAGAGAGCGAGAGCCAAGAGAGAGTC10.6172839506172839No Hit
TGTGTGTCCTCTGACTCCCTGAAACCCTCACACACTCCCAGGTAGACTAA10.6172839506172839No Hit
GCTTTAACCCACTGCGCCACCCAGGCGTACACCCCTGGTTTTTTTTTTTC10.6172839506172839No Hit
GGTAATAACAACATGAAAAGGTATTCAACATCATTCATAAGCAGGGAAAT10.6172839506172839No Hit
ATAATACCTCTTTCATCCAACAATAAGTGAACAACAAAAAGAGATCACAT10.6172839506172839No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC10.6172839506172839No Hit
TTGCATTGCTGTGAAAAGCACCCCAAGGTCATTTTGCAGATTAGCAGTCC10.6172839506172839No Hit
CTTTTTAGTTCCGCTAGTTCCTACCAAAGAATCCAAATCTTCCCAGACAC10.6172839506172839No Hit
TCTCCAGCAAGTCCCAGAGCTCCTGAGTCACAGCTGATGTGTGCCTTCTG10.6172839506172839No Hit
GGCCCTTTGATTCTGCCGGGCTGATGAATGTCTTCTTGGGATTTAGATCC10.6172839506172839No Hit
GTCCTAATTAGAGGAAATTGCAGATTCTCCAGCTAACCAGAATGGCATGC10.6172839506172839No Hit
GTCCCAGCCTTTAATTGACAAAGGCACACTGATGTCCCCAGATCCTTTGT10.6172839506172839No Hit
CCCTGATCCTTCGGGGAAGCGAACCGGGCACCCAGCCGCCCTGGCCCCCC10.6172839506172839No Hit
GAAATGGACAGATTCTTAGAAACAGACAAATTGCTGACTTAACAAATAGA10.6172839506172839No Hit
GCTGGGACAGAGGAAGCAGCAGGGGCAAGGCTGGAGATTGGATAAAGGCT10.6172839506172839No Hit
GCAGGAGTAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGA10.6172839506172839No Hit
ATAAAGACGTCTTTGAGGACTGATGGCCATCACCAGGTAAAGTCCTTCCC10.6172839506172839No Hit
GAAGAAAACTGGAAATTCATAAACACAGAGACTAAACAACATGCTACTGA10.6172839506172839No Hit
ATCCTCTTCCCTCAGCAGGTCTTGATGTAGGAAGATGTGTTTGGTGCGTG10.6172839506172839No Hit
GTTTTACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCAC10.6172839506172839No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA10.6172839506172839No Hit
TTCTAATTCTGTTTCTAGGAGGTGGGCGACTGGAGATACCTCGCCGCGTA10.6172839506172839No Hit
AGGGAGGTCAGCCTACACCCCTCCCTTGGTTTTCTCATTCTTCAGGAAAT10.6172839506172839No Hit
CATAACACATGTCTACTGGGACCTGGGGACAGTAATAAGGCAGAAGAAGT10.6172839506172839No Hit
CCTGTGAATTCTACGAATCATCCACAGAAAAAAATGAATACCAATCCTTC10.6172839506172839No Hit
GGCTATGACAGAGGAAATGTGGGTGCTTTGGAAGCCCAGAGGAAGGTGCC10.6172839506172839No Hit
CTGTAACTCCAATGCTCATGTCAGCCGATTAATAAATACCGGAAACTACA10.6172839506172839No Hit
GTGCGCCAGAGCACTCCATATGGAACGGAACAGATCACTAGTAGCTCTAC10.6172839506172839No Hit
CTATCTAAACATCTACAGTATGTTTTACCATTAAATGGGTCCAATAAACA10.6172839506172839No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT10.6172839506172839No Hit
GCCTGGAACACAGGGGAGCTCAGTGAGGGGCACGACCCTTGCAGCTCTGC10.6172839506172839No Hit
GTGACTCAGGAGCTCTGGGACTAGCTGGAGAAGACCCTTAGCCTCTCAAG10.6172839506172839No Hit
GGTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGA10.6172839506172839No Hit
GCTCCCTGCTGAGCCAGGAGCCCGATGTGGGGCCAGATCCCAGGACCCTG10.6172839506172839No Hit
ATCTTGTGCAGCAAACCAGCACTACCAACAGAAAACAAACAAAATAAAAT10.6172839506172839No Hit
CTCTGTCTTCTCTTCTGTCTCAAACCATCCTCTGCCTTGCTTTTATAAGG10.6172839506172839No Hit
CTGAAAAACATACCCTTAGACGACATCTATATTAGCTGTCAATGCCATCA10.6172839506172839No Hit
CGGTAAGGAGAGGGGAAGAGAAGGGGAGGGCAACCAGCAGGAAGAGTGAA10.6172839506172839No Hit
AGGGTGAGCCATCCTGGCAGAAGGCACCCATCAGCTGTGACTCAGGCGCT10.6172839506172839No Hit
CATCTTAATGGGCTCCCATCTCACCCAAAGTAGGGACAGAAGCTGTCACC10.6172839506172839No Hit
GGCTCATAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAAT10.6172839506172839No Hit
AGGTAAATGAAAAATTAATACAATGAAACAAAATTCACAGAATGCAGTAA10.6172839506172839No Hit
GAGCAGGAGTTGGGGAAGGCATCTTGAGAGCTTTTGAGTATCAGATTCCA10.6172839506172839No Hit
CCAACCATGTGATCAGAGGTTTGGAACTTCTAGCACCCCGTTCCCCAATC10.6172839506172839No Hit
ATGCTAAATCCTTCCACATTAAGATATACTTTCTTTCAAATTATTGTAAT10.6172839506172839No Hit
AGTGTAGTCAGGTTCTGGGCACATTTTGAGGGTAAAGCCAAAGGGATTTG10.6172839506172839No Hit
GTACTGACCAGTGTTGTTGGTTAATCGATGTGGTTGACTCTGCTGCTGTC10.6172839506172839No Hit
GTGCATGCTGGTTCACTAGGCTGGGGCCCTGGGAACCTCCAATTCCAGAA10.6172839506172839No Hit
GGCTTAACCACAGGGAGACACAGAGATCTGTCATGAAGATTCCTGGCTTG10.6172839506172839No Hit
TCCCAGTAGTGTGCCCCCACTTTCCATAGCTGGAGAATGTATTCATCACT10.6172839506172839No Hit
GCCTGAAGGCAGCCCCACTTCTGAATTTTCAGAATGTCAGCAAATAAATT10.6172839506172839No Hit
GAGAAGACACAGCTCCTTCAACAGCTGATGTGTGTGTAGGGCTTACTCTG10.6172839506172839No Hit
CTGGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCA10.6172839506172839No Hit
GCTTAGGACCCTATGGCTCATGTCTCCTAACATACCTCTAATAGTACAAG10.6172839506172839No Hit
GGTACAGGGTGTGGGGACCAGCCAGGCGGCAGAAGAACAGGTCGGGGTGG10.6172839506172839No Hit
TAGACGATTTGTCAAAAATGCCCGAAATGGGAAAGGAGCCCCAAACAACA10.6172839506172839No Hit
ACAGAGCGTTGTAAGACGCTCGTAGGCGTGCAGCCGCGTCAGCGGGTGGG10.6172839506172839No Hit
GCAGGGAAGGGCATAAAAAATTAGTGAAGGAAGATCAATGTGACATCGTG10.6172839506172839No Hit
GGTAGAACACCGTGTTCCCAGGAAGGTCTCTAGAAGGAAGAAACTTGGCA10.6172839506172839No Hit
AAACTGCGCAGTCAAGAATAGTAAGTCATCTGTTTGGGTTCATCCTGCTA10.6172839506172839No Hit
GCTCAAGACTTGTGCTCCAGACTTGTCTCCGTGCATCTTCCAAACCACCT10.6172839506172839No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers