FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1911-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1911-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992078
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG700.001056736241.427789
CTAGTAC1707.810195E-834.1238753
TAGTCGG2008.953066E-932.6243748
GCGAAAG2755.456968E-1231.642142
CGAAAGC3350.030.3040393
TATACTA1753.7736554E-629.0009085
TCTAGTA2853.2196112E-1027.9875642
AGCGAAA3255.0931703E-1126.7862721
GATATAT5750.026.4951131
GTATTAG3355.4618795E-821.6555671
TAGTACT2959.555357E-619.6626224
GTGTTAC5356.548362E-1118.984041
GTATAGT3451.7314069E-618.9250811
AGTCGGT3451.7423026E-618.9126829
TATAGAT4303.733112E-818.5498962
GTCCTAT11450.018.3741341
TAGTACC5158.039933E-1018.3006219
GTTACAT3201.9248815E-518.1283073
TATAATC2851.6307161E-417.8102682
TCCTATG11850.017.7458552
Produced by FastQC (version 0.11.5)