FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1911-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1911-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575352
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC1604.591493E-836.250975
ATACGCC1458.702991E-734.999826
AGCGAAA6400.032.864961
CGAAAGC7100.028.594133
GCGAAAG7550.026.8906962
GTGATCG1958.759009E-626.0255099
TATACTG8150.023.1294545
CTATATA5150.022.5270182
GTATATA8150.021.3583981
GTATAAG7500.021.2753371
GTTATAG4451.1277734E-1021.1884121
GTCCTAT16500.020.6599351
CCTATAC5301.8189894E-1220.520723
GTACATA6150.020.0488221
TACGCCA2659.3543924E-519.1508467
GTAGTAC2359.197701E-418.5123083
TTATACG3703.399391E-617.6361664
GTCTAGA7500.017.4070931
TGTACCG2550.001591258417.059285
ATACTAC6404.5474735E-1116.9921046
Produced by FastQC (version 0.11.5)