FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1911-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1911-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435682
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT18810.13101787164567083No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG17580.12245051480759667No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT14610.10176348244249074No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA14450.10064902952046484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG7950.038.301679
AGCGAAA8800.035.437041
GCGAAAG9600.033.2324452
GTCATAG9700.032.8898432
CGAAAGC9650.031.5564213
CATAGTG10050.031.02084
AGTGATC10600.029.4102087
GTATACG1000.00607446329.0008434
GGTCATA12650.026.945051
ATAGTGA12050.026.4737995
CGAATTA1100.00967323426.3634827
GTGATCC12100.025.7643158
TAGTGAT13050.025.0007276
TCATAGT14800.021.555433
ATAAGCG1700.003091673121.3256342
ACCTACG1700.003093547421.3234067
TCCGACT11200.020.7148885
GCATCGA4652.055458E-1020.268702145
ATCGAAT2155.057806E-420.23244145
ATAATAC7900.020.1911643
Produced by FastQC (version 0.11.5)