FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1911-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1911-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800156
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGTG3450.033.6251754
GCGAAAG2653.6379788E-1232.834182
TGATCCG3400.031.9851849
AGAACCG2802.6193447E-1028.4838245
CCGTAGA1555.4945165E-528.0679261
TAAGCCG1050.00770532727.6206785
GTGATCG1658.416704E-526.3635469
GTAATAT2351.3284753E-624.6838511
ATAGTGA4700.024.6823085
AGCGAAA3301.7680577E-924.1696031
TATATAG4201.8189894E-1224.1680935
CGAAAGC3352.1045707E-923.8088653
ACCGTGC2151.865183E-523.604578
TCCGACT3503.4960976E-922.787065
AGTGATC6050.022.7685187
GTATAAG2252.648199E-522.5582961
TTCCGAC3604.840331E-922.1540854
GTTAGAC2653.774372E-621.8894523
GTTATAC2353.704512E-521.598373
TAGTGCA3157.278268E-720.7142149
Produced by FastQC (version 0.11.5)