FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1911-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1911-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712179
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC9650.13549964264602019No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7210.10123859310650833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA700.00105611441.4311375
GGATAGC3400.040.517225
GTCGGTA2500.037.699688145
GGTACTG2850.035.6162455
TATACTG3700.035.2724575
TCGCCAT3900.033.461266145
GTATAGG5700.033.0884861
GATAGCA4200.032.7973486
ATACGGT950.004726581730.5260646
TTATACT4050.030.4339854
GCGAAAG2401.5752448E-930.216572
CCTTATA4100.028.30042
TATAGGT7500.026.1071192
TATGGAT5700.025.4455342
TAACTAT2301.0970616E-625.2313561
ACTATAC2901.1868906E-825.0050623
TGGATAG5600.024.599744
ATAGCAA5750.023.9563247
GTACCGA3702.6011548E-1023.5133197
GTTATAG1550.001802165523.400051
Produced by FastQC (version 0.11.5)