FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1910-rep2-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1910-rep2-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046309
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT10450.031.9286821
GTGATCG2252.8177965E-828.999599
GACGTAT1358.083632E-426.8514737
GTCCTAT11100.026.7917041
GTATATA4900.026.6450081
GCTATAT3306.184564E-1126.3758661
GTATAGG3151.027729E-925.3292061
CGAAAGC3601.8371793E-1024.1709443
GCGAAAG3952.3646862E-1123.8695472
TCCTATG11250.023.2085442
GTGTAAC3604.822141E-922.1630551
TATAGTA3604.827598E-922.1609342
TATATAC7000.021.753853
CTAGTAC2353.704331E-521.5995663
GGTATAT2704.4273474E-621.491451
CTATGAT13500.020.9451484
AATACCG1750.003659142620.7139916
CTATTAC2505.9535476E-520.3094181
TATACTG5002.5465852E-1120.2997115
ATACCGA1800.00430755720.1386037
Produced by FastQC (version 0.11.5)