FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1910-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1910-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336740
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC15890.11887128386971288No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14390.10764995436659336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1255.187758E-946.411961
TATGCCG750.001483322638.6679535
TAGTCGG1701.8299033E-938.3821958
GGTCGAA900.003624088132.2305261
GCGATTA900.00362873932.222088145
TATACTG5500.031.6374155
GTGATCG1453.4913635E-529.9998769
GTTATGT7200.029.2089161
GTATTAT13750.025.3156171
GTAATAT4700.024.6872141
GTAACGG2651.373628E-724.6289881
TAGTACC4850.023.918324
TTACGTG2502.2765526E-623.2042432
GTTGACA10950.023.179491
TAGCATG14500.023.0042082
TATAATA13000.022.8695682
ATTATAC3854.274625E-1022.5993
TACAGCC12550.022.5297877
CCGACTA3253.9910447E-822.3084344
TATTATA5300.021.8907972
Produced by FastQC (version 0.11.5)