FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1910-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1910-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823045
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9070.11020053581517414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT651.0913012E-555.7879522
GCACGGT1157.13961E-637.827296
TATACTG3550.036.7639665
GGTCGTT1051.8537462E-434.5228167
GCGATTA1051.8537462E-434.522816145
TTACCGC1153.154274E-431.5342391
CGGTGTA1402.7466322E-531.070539
CTACTGA8300.030.5732636
TATAACC3251.8189894E-1229.0079733
TAACCTA2104.980611E-727.6216075
GTATTAG1358.062794E-426.86251
TACTGAA9450.026.8510747
GTTGACA6500.026.7798461
GTATTAT7300.025.8321591
ATATAAC2901.1857992E-825.0083942
GCGAAAG3501.2914825E-1024.8654882
GGATATA2401.592005E-624.1762491
TAGTACC3301.7680577E-924.1703724
AGCGAAA3402.4920155E-923.4651831
ATAACCT3753.074092E-1023.203564
Produced by FastQC (version 0.11.5)