FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1910-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1910-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691178
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA9590.13874862915196953No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9020.13050183888954797No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT8070.11675718845217875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGT400.00605037254.374389
AGCGAAA2150.047.2258451
GCGAAAG2150.047.2121772
GTTACGT657.322356E-444.6310161
GTTATGT4850.041.8703351
GTACTAG750.001480517938.6802141
TAGTCGG1209.560328E-636.2495848
CGAAAGC2850.035.6162033
CGCACCA1301.6533555E-533.4635775
CCGACTA2257.912604E-1032.2241824
TATACTG2752.1282176E-1029.0017665
AACAGCG1000.006073517728.9996689
CTACTGA8650.028.4988446
TTATGTG7750.028.0662232
TGTTGGC8800.028.0110447
GCACCGT4950.027.8299756
TAGCATG6800.027.7222732
GTATTAG1855.7969155E-627.4420451
CCCTATA1606.808873E-527.1970271
AGCACCG5450.026.6071225
Produced by FastQC (version 0.11.5)