FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11803523
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1118730.947793298661764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC17350.032.520293
TATACTG37150.029.9861855
GGATAGC26750.029.205145
ATCCAAT55700.024.9205117
TATAGCG4450.024.3832952
GACAGTA29800.024.2602127
CAGGTAC25550.024.0650833
GCAGGTA26850.023.9776652
CCTTATA40200.023.3925972
ACTGATC31650.023.2990048
GTATGGA39000.023.1097851
TTGGACA34950.022.973924
TATGGAT34350.022.7435472
GTATAGG15100.022.6234951
TGGATAG35150.022.0200234
AACGAGG32200.021.244457145
AACAAGG68750.020.6107737
ATACTGG45800.020.532286
GTATTAT30800.020.530871
ACCAGGC71850.019.5202718
Produced by FastQC (version 0.11.5)