FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_Stock-Control-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_Stock-Control-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528999
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT55481.0487732490987696No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA52820.9984896001693765No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG51840.9799640453006528No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG51620.9758052472688985No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG43780.8276008083191084No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT42520.8037822377736062No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC35230.6659747939032021No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT27390.5177703549534121No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA27310.5162580647600468No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT25040.47334683052330906No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA24870.4701332138624081No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA23270.43988740999510395No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT20650.39035990616239347No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA20450.3865791806789805No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT20240.3826094189213968No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC18670.3529307238766047No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG18620.3519855425057514No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC18510.34990614348987426No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG18090.34196661997470695No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT16820.3179590131550343No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA15780.29829924064128666No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT14650.27693814166000313No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC13960.26389463874222824No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG13600.2570893328720848No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC13260.25066209955028274No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG13040.24650330151852837No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT12920.2442348662284806No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC12710.2402651044708969No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA12190.23043521821402307No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC11720.2215505133280025No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT11380.2151232800062004No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA10810.2043482123784733No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA10520.19886616042752445No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC10420.19697579768581794No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA10390.19640868886330598No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC10320.19508543494411143No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG10000.1890362741706506No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC9930.18771302025145606No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA9680.1829871133971898No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT9440.17845024281709418No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT9240.17466951733368116No Hit
ATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAGACGTA9230.1744804810595105No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA9160.17315722714031595No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT9130.172590118317804No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG9050.1710778281244388No Hit
ATACTGAGGACCCAGATGAAGGAACAGCAGGAGTAGAGTCTGCAGTATTG9040.17088879185026815No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG8880.16786421146353772No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA8830.1669190300926845No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA8820.16672999381851383No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG8760.16559577617348994No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG8720.16483963107680732No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA8710.16465059480263666No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA8670.16389444970595407No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT8590.1623821595125889No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA8520.1610589055933943No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA8410.15897950657751714No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA8370.15822336148083457No Hit
GATATGGACCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGG8350.15784528893249328No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT8350.15784528893249328No Hit
GGAGTAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGA8250.15595492619078674No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA8090.15293034580405634No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG8000.15122901933652047No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA7970.15066191051400854No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG7940.15009480169149658No Hit
GTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACC7880.1489605840464727No Hit
ACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAA7800.14744829385310748No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT7790.14725925757893682No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG7770.14688118503059552No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGA7710.1457469673855716No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG7660.14480178601471838No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG7630.14423467719220642No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT7600.14366756836969446No Hit
TGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGG7550.1427223869988412No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA7510.1419662419021586No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT7480.14139913307964663No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT7380.13950877033794015No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC7370.1393197340637695No Hit
TTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACCAGCATTAAGCAT7330.1385635889670869No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA7310.1381855164187456No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC7310.1381855164187456No Hit
TTACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCT7250.13705129877372169No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT7190.1359170811286978No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA7190.1359170811286978No Hit
TAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTG7070.13364864583864997No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT7030.13289250074196737No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC7030.13289250074196737No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA6980.13194731937111412No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG6890.13024599290357827No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT6880.1300569566294076No Hit
GTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTTAAGAA6800.1285446664360424No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA6790.12835563016187176No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG6740.1274104487910185No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC6710.12684333996850655No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC6670.12608719487182396No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG6610.12495297722680004No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC6610.12495297722680004No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA6540.1236297233076055No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA6490.12268454193675225No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA6360.12022707037253379No Hit
ATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTC6180.11682441743746208No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA6170.11663538116329143No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA6160.11644634488912077No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAAT6090.11512309096992622No Hit
GTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGGA6080.11493405469575556No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG5990.11323272822821973No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG5950.11247658313153712No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA5920.11190947430902516No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT5910.11172043803485451No Hit
ATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCA5860.11077525666400125No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT5810.10983007529314799No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG5810.10983007529314799No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA5750.1086958576481241No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG5750.1086958576481241No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA5740.10850682137395345No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA5640.10661645863224695No Hit
CATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAAT5620.10623838608390564No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT5610.10604934980973499No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT5550.10491513216471107No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT5500.10396995079385785No Hit
TTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACCAGCATTAAGCATC5480.10359187824551654No Hit
TCGTAAGATTGCTCAGTTCATTGATGCTTAATGCTGGTCCATATCTTTTG5450.10302476942300458No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA5420.10245766060049263No Hit
GAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAG5410.10226862432632197No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA5350.10113440668129807No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA5330.10075633413295676No Hit
GCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGGATTAGAAATC5320.10056729785878611No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT5300.10018922531044483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA4600.0119.782382
AGTAGAA7200.0111.8023151
CAGGTAC5500.0100.1816253
GCGAAAG1400.098.392672
GGTACTG5750.095.82595
CGAAAGC1450.094.999823
TAACTAT554.1472958E-1092.298721
AGTTTTA259.384359E-486.99983145
AGCGAAA1650.083.508371
GTACTGA6650.082.856986
AGCAGGT6800.081.052241
ACTGATC7200.080.55548
CTGATCC6950.080.3235869
TATACTA702.8085196E-972.499865
CTAATAG502.3047633E-672.499864
CCTCTGC502.3047633E-672.499869
ACAAGGC4650.070.161158
TTTTTGG11350.069.6451641
GACAGTA11450.068.384157
TTTGGAC11550.067.7920763
Produced by FastQC (version 0.11.5)