FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_Negative_Control_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_Negative_Control_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTATATTGTCCCTCTGGTTCACGGATCTCTGCACTTGTTTTCTGAAG40.16019223067681218No Hit
GCTCTGAACTGTGCCTGGGACCATCTGTACTTTATCCTGATTTATTTTGT40.16019223067681218No Hit
AGCCTAGAGATTGCCGAGGAGACCAGTGTTATTATGGAGTGGAGGAAGGA40.16019223067681218No Hit
GTTAGATGCTTAACCAATTGAGTTACCTAGGTGTCCCCATGGGGTATTTT40.16019223067681218No Hit
GGCTCAGAGAGAGGGGCTGGCAGCCAGGCTCAGGCCACTGTTGGTGGGCA40.16019223067681218No Hit
GGGTGGGGAAGAGTGGAGAAAGAGAGAGAGGCCTGCATGCTCCTTTCCTG30.12014417300760913No Hit
TCCCTGCAGAGGGTCAGGGGATGGGTCTGGCAGTACCATTTGGGATGCAC30.12014417300760913No Hit
ATGTCAAGCACTGATCTGGCCATCTCCTTGTTGGGACACCGTGCTAAGCC30.12014417300760913No Hit
GTTCTGGAGTGTCCCCCTGTGTGGCTTGCAGCTCTTCTCCAGGAGTCCTC30.12014417300760913No Hit
TCTTACACCAGCTATGTGAGGGAGGTAGTTCATAGGTGAGAAAACTGAGG30.12014417300760913No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA30.12014417300760913No Hit
GAATGAGGGCGCCTTGGCAGTGAGCTCGGGCTTCTGCCCTGCACCTGGTG30.12014417300760913No Hit
GACATAGCTTTGGTTAGGGAGGCCCATGCCCGAGGGGAGAGACCCTCGTA30.12014417300760913No Hit
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA30.12014417300760913No Hit
ACCCAGGAGATGGAGAACCCAACGGAGGGGGGACTAGGAGACGAGCAGGG30.12014417300760913No Hit
CATCTAACCTCGGTGGTGGGTGGGTAGGAAAGGAAAATGTGTCTTTTTTC30.12014417300760913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG350.003459335320.714285140-144
Produced by FastQC (version 0.11.5)