FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_Negative-Control-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_Negative-Control-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCCAGTTCTGTGGAGTCCCCCGAGGGAGGGAGACCGAGTGGGCGGCC10.9615384615384616No Hit
TTCCCACACAGATCCTCGTGAGAATCCGAGAGCTGATTTTAAAAGGCCAA10.9615384615384616No Hit
GTCAGACACAGCCTTCCTCTGAGGACACAGGGACGATGGTGTGCAGGTGG10.9615384615384616No Hit
AATACATATGTCTTTAAAAATCTTATCCTGAACATTTGGTGTTGCGCAAT10.9615384615384616No Hit
ATCATGACCTGAGCCGAAGGCAGCGGCTTAATCCACTGAGCCACCCAGGC10.9615384615384616No Hit
ACGAGAAGCAGTGTCTACGAGTTCAGAGACCGCAGCCGCATAGCCATTGG10.9615384615384616No Hit
GACCAAAAGAATTCGGAAGGCCAAAAATAATTTTCGAATATTTTAAAAAC10.9615384615384616No Hit
GCATTTCATCAAGTGGAAATCCAGTGGCAAAGGAGAATTAATGTAGCCTA10.9615384615384616No Hit
AGTTTGATTTGTGGATGTTGAACCACCATTGTATCCCTGAAATACATCTT10.9615384615384616No Hit
GCAAAATGTCCTTTAAGAACAAAAACTGGGCACATTCTAAAATCAACACA10.9615384615384616No Hit
AATTGGGCAAGGTGCACGAGCCTCCGTTGACGAAGGACGCCATTGCTGGT10.9615384615384616No Hit
GCATGAGGTTCTTGAGGCCCTGGAAGGTGTCGGGCTTGAAGACACCATCG10.9615384615384616No Hit
GAATAGTACCTATGAGAGTAAGCCCTCAAAAAATGTTGTAAAAAATCTTA10.9615384615384616No Hit
ACAATAGAATTCTACCCTTTCGTAATATATCGAAGTACATTGATGCATAA10.9615384615384616No Hit
GGTATATGGGGTGTGTGTGTGTGTACAGGCATGTGTGCAAAGAAACCAGA10.9615384615384616No Hit
TAAGAAGAGATTTTAAAAATTCCATAAAGAGTCATTCCATGTAAGGTTGA10.9615384615384616No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA10.9615384615384616No Hit
TATATGACGATGTAGTACAAAGCTGACAGTGAACACTTTTACAAAATCAC10.9615384615384616No Hit
CTTCCCTCAATGATCCCCCATCCAGACAGAAGATCACAAAGGAAACATCA10.9615384615384616No Hit
ACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTC10.9615384615384616No Hit
TGGTTCTGCTTATATGTGGATGTTTTATGGTACAGCTGTAAGTGTATTTT10.9615384615384616No Hit
CTCTCTCTCTGTTAAAGAAATAAACAAAATCTTAAAAATTGTTTGTAGAG10.9615384615384616No Hit
CCTTCGTTTTGTGAATGCGGCCCATTAGTTTGATTTGTGGATGTTGAACC10.9615384615384616No Hit
ATGTTGTTTAGTCTCTGTGTTTATGAATTTACAGTTTACTTCTTGTAATT10.9615384615384616No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC10.9615384615384616No Hit
GAAGAGACCTAAACCAGAAGAGAATGAAGACAGGTGTGCAGAACCATTGG10.9615384615384616No Hit
CATACAGCACAGCTGACCTTTTCTGAGTGTGTCGTTCTAAGCTTCAACCC10.9615384615384616No Hit
GTAAAAGACAGAAGGTAGAAGGAAAAAAGGAGAAAAAAAAGGACAGGAAA10.9615384615384616No Hit
CCTCTCCGCAGAGACATCCCTTCCCGAACCAGACAGGTCGGTATCCTCAC10.9615384615384616No Hit
TTCCTGCCCAGCAGGGGAAACCCAGAGTTAAACACAGCTAACAGGAAAGC10.9615384615384616No Hit
AATAACAGATCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACAG10.9615384615384616No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC10.9615384615384616No Hit
GAACAATTACAGCCCAACAAGATGGATAATCCAAAAGAAAAGGATAAATT10.9615384615384616No Hit
GTATCAATGTCACCGGGACAACTCTGACTTGCGATTCTTCCTTTAAGAAG10.9615384615384616No Hit
ATATATAGTAGCATTGTAACAGCCATTAAAAACAAGATGTGGAACATTTC10.9615384615384616No Hit
GAAGGGTGGATTTCAAATCATTTTTAACAACACGGCACTTCTTTCAAATA10.9615384615384616No Hit
GTGTCCACCCTCAGAGATGGAGTCTGGGGACAAATCCAGGCACTGGCAGT10.9615384615384616No Hit
GTGAATATATATTAAAGAATTCAGTCTTGATTGTATTTGTCTTTATGCCT10.9615384615384616No Hit
CAGCATCAGTGAGGTGCTCTCTGGGCCATGCTGGCCTCACTTGACCGCCA10.9615384615384616No Hit
CCTTGTGCAGTCTTTTGCAGGGCAAAGTGTACCTTCCCCCAGAAAAATAG10.9615384615384616No Hit
GTTTATAAATACGTCTGGCAAGGCTTTATGTTTATGCATGGGTCTATGCA10.9615384615384616No Hit
TTTATTGACTTGTCTTGTGGCCTTACATATGACCTAAGTTGGAGAACGTT10.9615384615384616No Hit
ATCGAGTGTAAAGGGAGAAAATGAACACATTTTTATATATGCAGATTTCC10.9615384615384616No Hit
CTGAGGGGCTGCGCCGCACAGTCCATAGAACTAAATCCCGAGTGTTGGCA10.9615384615384616No Hit
CACACATGAGGACCTGGCAATCATTGCCCGGTAGGCCGCACCCGTCATCC10.9615384615384616No Hit
CCATTAGCATATGGACATTAACCTAATAGGTAGACAGATGGGTGACCAAA10.9615384615384616No Hit
GTATTAATATGCATGACCTTGTTTAGCCTTGCAATGGCCTTGTAGAGTTG10.9615384615384616No Hit
CCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCGGCCCTGCCCC10.9615384615384616No Hit
CCCACTTATGCTAACACCAGGAGTTGTAAATACTGAGAGCGAGGTCCCCA10.9615384615384616No Hit
CCACTGAGCCACCCAGGCACCCCTCATCTAGTGCATTTTAAGTAGTATAT10.9615384615384616No Hit
GTCTGAAAACAACTGAATTGTGGGATCTCTAAGGACCCTTCCAGATGGAC10.9615384615384616No Hit
TATCATAACACGTGAATCATTAACTGACACTGCCACAAATCAAAAGTCTT10.9615384615384616No Hit
GTTTCAAACCCAAACAGGCTGTCTCCAGACCCCATGCTCCTAACTCCTGC10.9615384615384616No Hit
GCCCTATTCCTAGTCCTTAAAGATTTTCTCCTGAAGCTTGTGTTTTGTTT10.9615384615384616No Hit
GTAGTTGATTGAATATATCTGTAGGTCACATCTTTTAAACACACAAATAT10.9615384615384616No Hit
GGACAGGACCCTGTACTAAAAGGATGGGAAAAATGACGTGTTGTGCCTAC10.9615384615384616No Hit
CACATTAGAGCAGGCGAAGAGCCGGCAAAGGGCTGATCAGAGAGGCTTTG10.9615384615384616No Hit
TTCTAACTGGCATGAGATGGTATCTCATTGTGGTTTTGATTTACATTTCT10.9615384615384616No Hit
GCTTCCCTTCAATGTCATTGCCATCATCAGTATGTTGCTGATCAAAAGAT10.9615384615384616No Hit
GGTTAGGAAGCTTGTAGGAAGTAGAACATGGGAGTCTAGAGAAAGCAGGT10.9615384615384616No Hit
TGCTGTGTGTGTGTGTGTGTGTGTGTGTGTGGGGTCTGTGGTGTATAGTG10.9615384615384616No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC10.9615384615384616No Hit
GGCACACACATGACATCCGCTAGAGAAGGGGACTGTTCCCAGAAAGTGAA10.9615384615384616No Hit
GCCTGAGATTAAAACAAAACCAAACCAAAAACAAAACAAGAACAAGAACA10.9615384615384616No Hit
TTATTAGACTTAGTGAGCACCCTGAATAGGAACATTAGGTCTAATTCTCC10.9615384615384616No Hit
GGGACAGGGTGGACCCCAAGGTCCCTGGCCCTCCAAGGCCCCATCATTCT10.9615384615384616No Hit
GCGTTAATAGCATGTGTTTGGTTTACTCTGTCATTTATGCCTGAGTTTCA10.9615384615384616No Hit
GACATACAAACATTCAGTCTATAACAGGGACACTGAGCCTGAGGGAGAGG10.9615384615384616No Hit
ATATATAGCTGATGGCACATTTCAGCAGACCGAGCAGGACTCTTCTCATA10.9615384615384616No Hit
CAATTGGGAGAATGAGTGTTCAGTGAATATTCCGCCCCTTCCTTGCCCTT10.9615384615384616No Hit
TCCCTAACCCATACCCTCTATCTGTCCCAACTCATTGAGTTAACTATTGT10.9615384615384616No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA10.9615384615384616No Hit
CACTGACGGGCATGTCTTTAATCTGCCCGTACCAGATTTTAGAGGCCCGT10.9615384615384616No Hit
GACCCTATCTCGAGGAGGAGTTGCTGCTCCAGTGTTCCGGATGCAGAATT10.9615384615384616No Hit
CCCCTTTTCTCCTGCTTACAGTCTTGGGTTGAGATTATTTGTATTTTTTT10.9615384615384616No Hit
GTGGTGGCGTCCCAGCCCATGAGGATATCGAGTACCGCCGATAGGTCGCC10.9615384615384616No Hit
GAAGATATCATTTAAAATCTAAACTCGAAAGCACCAATTCCAGACTAAAT10.9615384615384616No Hit
GTTTTAAAGTAGGCTCCACACCAATTGTGGGGCTTGAACTCACAGCCCTG10.9615384615384616No Hit
GCCCTGCGGACCCCTTCATCTCAGACTTCTGGCCTCCCGCACTGAGAGGT10.9615384615384616No Hit
TCACTGTTTGCATATAACACCCAGTGCTCCATGTAATACTTGCCCTCCTT10.9615384615384616No Hit
GTATTGTTGGCCTTTAGCATTCTCTAATCACAACCAAATCACATTGCACT10.9615384615384616No Hit
ATTTCAGGCTTCGGAAGCACCAGGGGTCAAATTCACGGCGAATGGGGTTG10.9615384615384616No Hit
GCTTCGGACACGGCCCAGTATCGCCTGTCTCTTCGGATAGTCGAGCTGGC10.9615384615384616No Hit
GCTTGTGGACTGCTGGCCACCATGGCTTCTTTGTGAAAACCACATGCTAT10.9615384615384616No Hit
GTTCAGTGACATTAAGTACGTTCACAGTGTTGTGCCCCCATCACCACCAC10.9615384615384616No Hit
ACAATAATGTACGTGATACGAGCGTATTTTTCATTAGTATAATTTAATCG10.9615384615384616No Hit
CTGCATGAGGTTCTTGAGGCCCTGGAAGGTGTCGGGCTTGAAGACACCAT10.9615384615384616No Hit
ATGCAGAGAGGGGCGACAACAATCATGCTGGGCGGCCACACTACAGCTCA10.9615384615384616No Hit
CTTTTATACACATCCTCGTATTGGAGTCAGGATATGGTCATTGGAGAAGA10.9615384615384616No Hit
GACATGTCCACACTTGTTCTCAGAGCTTTCTTTGCAAAGGAGGGCATTTC10.9615384615384616No Hit
GACCAGCACAGATAACATACTAACGTATGCTTCCTAGAATGAGAAGGAAG10.9615384615384616No Hit
CAATGCAGATGCTTAACTGACTGAGCCACCCAGGCGCCCCAGGATTTCTA10.9615384615384616No Hit
CTGGGGGGTGAAGGTGGTCCGGTACTCAAAACACTCACTGGGGTGTCGCT10.9615384615384616No Hit
AGTATAGACTGGTGCTCGAGGGAGTGTCTGGCTCCGGTTCCCAGCCACGA10.9615384615384616No Hit
CGAGAGACCTGCTGCTCCCACCCCCTGGACACTCTGGCGTCGAACCATAC10.9615384615384616No Hit
CCTTTCTGGAAGCTCTAGGAAAGGATGCATTTCCTAGCTCACTGGAGTGG10.9615384615384616No Hit
AATTATTGGAAAGGAAGAGTCAAAATGACTGTTATTTGCAGACAACATGA10.9615384615384616No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG10.9615384615384616No Hit
GGCTTAACCACAGGGAGACACAGAGATCTGTCATGAAGATTCCTGGCTTG10.9615384615384616No Hit
GCCCTGAACTCGCAGAAACCCCTCCTGGAGGAACGGGCAATCAGATGCCG10.9615384615384616No Hit
CGATGGGAGTGGTGGTTACTGTGACCACCACTGGCTCCTTCTGAGAGCCC10.9615384615384616No Hit
CATTAATGAGCCCGTGCTGGCCACGGGCATCAAGGCCTCGAGGCAGAAGC10.9615384615384616No Hit
GAACCACCCTGGGCATAGAGCTTATTAAGCCAAACCAAAACAACCCCCCC10.9615384615384616No Hit
CATTATTACAGATAACATTCCATTTTTCACCTCCAGGCCTATCCTCAAAC10.9615384615384616No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers