FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_Neg-Control-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_Neg-Control-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTCAGGGTGCTGGCCTGCGCTTCGTTTCTGGCGTTCTCCCGGCC11.5625No Hit
CTCTATGCCCAGTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCA11.5625No Hit
CCATGGATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGTTG11.5625No Hit
ATGTAACACTATTTAGATAAATCTGATAAAAAGTACAAGTAATATTGGTC11.5625No Hit
AAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGT11.5625No Hit
GGTGTTGGTAGCAGCGGTGACCCAACTGAAAGGTCATTGGGGCACTGGCA11.5625No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA11.5625No Hit
GTACAAGAAAGAGAAGATCAACCCGTTGGGTGGATGCTTGCCGATTCTGG11.5625No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC11.5625No Hit
GGAGCAGGCAGCAGCCTAGCCAAGTAAGGGCAGTATGCATGTTTTAGCAG11.5625No Hit
ATCTGAGGGGACGTGGTGTTGTCTTCAGAAATGGCTCAATTCTGGCGTTC11.5625No Hit
CCCATACAGGTTTGTCTTCCTTCGTCCTTCTTTTGTTCTGCATTTGCATA11.5625No Hit
GTCCCATGGCAGATCCCATAGCCCTTCCGAAGCAAACCTCGCTGCCAGAT11.5625No Hit
GCTTTCCGGAAAAACATGTCCCTTTTCCTGTGGCATGAGCCGGAGGTATT11.5625No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.5625No Hit
GAATATACCAGAATACCCAGACCAGTTATCACTGTCAACTATGACTTGCC11.5625No Hit
CCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAAT11.5625No Hit
ATCTAGTTGATCAAACAACTAAATGTTGAGAAAAACTCTATATTTTAATC11.5625No Hit
GGATTGGTGGCTCCGGACTACTGGAGTTGAAGGGAAAGCTGGTTCTGGCA11.5625No Hit
ACATAAGCCCTGAGGGAATAATTTAAAAGTTGATTTGCACTTCATCCAAA11.5625No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11.5625No Hit
CTCTTGATTTTGGCTCAAATCAAGAACCTAAGCTCCTGGGAGAGAGCCCC11.5625No Hit
ACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCA11.5625No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC11.5625No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA11.5625No Hit
CCTGGAGTTCTTCTTTCTTCACTTTGGTTATCGTTAGTTGCGATTCCGAT11.5625No Hit
CTATTCCGGCTCAGGATCTAATGGGCTCCACAGAAGACGGGCCTTCAGGA11.5625No Hit
ATGTGCAACATCCTCAAAGGGAAATTTCAAACAGCAGCACAAAGAGCAAT11.5625No Hit
GGATACACCATGGACACAGTAAACAGACACCAATACTCAGAAAAGGGAAA11.5625No Hit
AAGATTCTCTCTCTCCCTCTGCCCCCTGGCCCCGCCGTCTCGCCCCCCCC11.5625No Hit
GTACATCGCCAGTGCCCGATACAGCAGGGAAGGCGTGCTTACGGTTTGCA11.5625No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC11.5625No Hit
GAACTAAAAAGTGTCCTGAGTTCCTCTAGGTTTTCTACATTCCCAGGGTA11.5625No Hit
AATAAACATAACTAATCATAAATTCTCAAAATATAACAGTAACAAAAAAA11.5625No Hit
GCACTGGCCTAGTCCCCTGTCCACCGGAACGCCAGAGCAAAAGGGCAGGC11.5625No Hit
GATTGCTGTGTTCCAGAGCACCTGGGGACTGTGAATTTGCCCATCAATGT11.5625No Hit
GGATAGATCCTTTCCGTCTGCTTCAGAACAGTCAGGTCTTCAGTCTTATT11.5625No Hit
ATTATCCAACATTTATAAATATTTACCGCCCCAGCATGGAGCAACTAAAT11.5625No Hit
ACTCTTACCTGTGCCAAGGAATACTTCACCACGTTAGGAATCCTAGTAAT11.5625No Hit
GACCAGTAGAGAGCTTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAG11.5625No Hit
GCGTATATAGATATTGTAAGGGTTTCGAGCAATTAAAATTCATGAAGAGG11.5625No Hit
AGATAATAGCACTTGGCTCTGTTTCTATAACTATTGCGACAATATGTTTA11.5625No Hit
GCTGCAGACAGCTTGACCACTCAAGAGTCACCCTCCTGTTCCCAAATGGA11.5625No Hit
GGAAGAAAGGGTAGGATCTGGAAAAGTGGACTCTGAGGGGTTGAGGGCGG11.5625No Hit
GCCTAAATTCTAATCTCTAAGACTATTATATCAGTTAATTTGTAAATTAA11.5625No Hit
CTGATAGGCAACTTCAGCAAAGTCTCAGTATACAAAATCAATGTGCAAAA11.5625No Hit
GCTAAATTGGCAAATGTTGTGAGAAAGATGATGACTAACTCACAAGACAC11.5625No Hit
CCCATCGACAATGTCATGGGTATGATTGGAATATTTCCTGACATGACCCC11.5625No Hit
CTCTGAGGGGCTTCTATTTTTGTGGGTTATACCTATCTATTCAATCAATC11.5625No Hit
GCTAGAAACAGCCTTTCCCATCTTCCATAGCATTGGAGACCAGTGCCCTC11.5625No Hit
AAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCC11.5625No Hit
GTTTCCTGTTTACTGCCCAGTGGACATCAATTACTCTCTGTTTGCTTTCA11.5625No Hit
TTGTCGAAGCACTAGCAAGGAGCATCTGTGAAAAACTTGAGCAATCTGGA11.5625No Hit
GGGCAACCACCATTCTAATTCTGTTTCTAGGAGTTGGGCTACTGGAGATA11.5625No Hit
CAGGTGGGTCCTGCCCTCAGGGAGCTCACTATCACTATCAACAGGGACAT11.5625No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA11.5625No Hit
CCTAACTTGATACCTCAAGGAAACAGAAAAGAACAAATAAAGTCCAAAGT11.5625No Hit
AGTGAATGCTGAGTGTTTTCCTGGTGGGCCAGCCCCTCTGCCTCCATGTC11.5625No Hit
CTCCTTCACAGCTTATTTTAAAACATCAGTCTTGTATAAGGATATGAGTA11.5625No Hit
TCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGGG11.5625No Hit
CTCCTTCATAATCCCTCAGAATTGAGGAGCCTGGAGAATATTCTGGGAAG11.5625No Hit
TTGTGGGGCCGTTTGGGCTATTTCACAGCAATCCCGTTGCTAGCCCAACT11.5625No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC11.5625No Hit
TCAACAGCCACTTGCTCCACTAAAAAAGAAAGAAAGAAAGAAAGAAAGAA11.5625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTTC50.0145.09
GGCAACT50.0145.07
ATAGGCA50.0145.04
CTGATAG50.0145.01
GCAACTT50.0145.08
TGATAGG50.0145.02
CTAGGAA50.0145.0145
AGGCAAC50.0145.06
GATAGGC50.0145.03
TAGGCAA50.0145.05
Produced by FastQC (version 0.11.5)