FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_HK_1073_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_HK_1073_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGCATCCTTTCCGAGAACTGTGAGTCTCTTCGTGGCCTTGTTATAGT13.225806451612903No Hit
GTTGCATACATGTTGGAGAGAGAACTGGTCCGCAAAACGAGATTCCTCCC13.225806451612903No Hit
GAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCATCTG13.225806451612903No Hit
CAGTTAAAGTGCCAGCATCCTTTCCGAGAACTGTGAGTCTCTTCGTGGCC13.225806451612903No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT13.225806451612903No Hit
ATCTCGTTTTGCGGACCAGTTCTCTCTCCAACATGTATGCAACCATCAAA13.225806451612903No Hit
GGTTCAAATTCCATTTTATTGTATAGCATTGTAGGGTTCTGGGACCACTG13.225806451612903No Hit
CAAGTATTCTCATTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAAC13.225806451612903No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC13.225806451612903No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA13.225806451612903No Hit
TCTCCCAACCATTGTGAACTCTTCATATCCCTCATGCACTCTTATCTTCA13.225806451612903No Hit
AGTCAAGAGAGAGGAAGAGGTGCTTACGGGCAATCTTCAAACAGTGAAGA13.225806451612903No Hit
GCTCTTCTCCCAACCATTGTGAACTCTTCATATCCCTCATGCACTCTTAT13.225806451612903No Hit
GGCTGGCAATATCCCAAGCATTCCCATCACATTGTCGATAGGTTCATCTC13.225806451612903No Hit
CAAATGTCCCAAGCACATCCCTCATTTGTTGGGACCGCGTTGTTTCAAAC13.225806451612903No Hit
CTGTGGCACATCTCCAATAAAGATGCGAGTGGATCTGCTGATACTGCAGC13.225806451612903No Hit
CTTCCTCTCTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCA13.225806451612903No Hit
CACCAAAACTGAAGGATGAGCTAATTCTCAGTCCCATTGCAGCCTTGCAT13.225806451612903No Hit
CTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACTGACAATAACT13.225806451612903No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT13.225806451612903No Hit
CGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAA13.225806451612903No Hit
CAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAGTC13.225806451612903No Hit
GTTCATTGATGCTTAGTGCTGGCCAATATCTCTTGTCTTCTTTGCCCAGA13.225806451612903No Hit
CCCACTGTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTT13.225806451612903No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT13.225806451612903No Hit
CAATAATAAAAACAAGACTATTCACGATAAGACAGGAAATGGCAAGCAGG13.225806451612903No Hit
CCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCA13.225806451612903No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT13.225806451612903No Hit
ACTAGCATCTTTATTGGAGATGTGCCACAGCACACAGATTGGTGGAATTA13.225806451612903No Hit
GATTGGGATATTGCCAGACATGACTCCAAGCATCGAGATGTCAATGAGAG13.225806451612903No Hit
GCTAGGAACATAGTGAGAAGAGCTGCAGTATCAGCAGATCCACTAGCATC13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)