FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_HK-1073-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_HK-1073-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748179
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT43850.5860896924399107No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT40790.5451903889309911No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC38830.5189934494285459No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA36710.49065798425243157No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG32430.43345242248178584No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG30410.4064535358517147No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT29890.39950332741229033No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA25630.3425650813508532No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT25530.3412285028048101No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC23810.31823935181286833No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC22310.2981906736222214No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA21710.29017120234596266No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG21530.2877653609630851No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT21390.28589415099862464No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG19990.2671820513540209No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC18170.242856321816036No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA17820.23817829690488507No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA17310.2313617463200651No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA17250.23055979919243924No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC16670.2228076436253891No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT15670.20944185816495786No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG13970.1867200228822247No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC13740.18364589222632552No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG13730.1835122343717212No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG13670.18271028724409533No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC13060.17455715811323227No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC13060.17455715811323227No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC13000.17375521098560637No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG12910.17255229029416758No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG12900.17241863243956326No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG12730.17014644891128994No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG12440.16627037112776488No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA12260.16386452974488727No Hit
GTACATTGCCATTCCACAATATCAGTAAATATGCATTTGGAACCTGCCCC12180.16279526690805277No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT11960.15985479410675787No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC11920.15932016268834062No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA11890.15891918912452768No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT11740.156914321305463No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA11700.15637968988704576No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA11620.15531042705021125No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11450.15303824352193793No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA11420.152637269958125No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC11350.1517016649758948No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG11150.14902850788380856No Hit
ATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTA11060.14782558719236974No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG10870.1452860879548878No Hit
GTGTAGTGCAATGTCAGACTGAAAAAGGTGGCTTAAACAGTACATTGCCA10840.14488511439107488No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT10710.1431475622812188No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC10710.1431475622812188No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA10620.14194464158977999No Hit
TTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTATTGATCAT10490.14020708947992394No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA10400.13900416878848512No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT10330.13806856380625493No Hit
ATCATATTGAGTCTAGTTTCAACCTCACTGAATTCATGATCAATTATTTC10240.1368656431148161No Hit
AAACAGTACATTGCCATTCCACAATATCAGTAAATATGCATTTGGAACCT10120.13526174885956435No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG10110.13512809100496004No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT10080.1347271174411471No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA10080.1347271174411471No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT9730.13004909252999616No Hit
ATCTTATTATTGATCATATTGAGTCTAGTTTCAACCTCACTGAATTCATG9620.12857885612934875No Hit
CTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGTTC9400.12563838332805385No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT9330.12470277834582366No Hit
GCACTACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGG9270.12390083121819777No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG9270.12390083121819777No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG9180.12269791052675898No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC9120.1218959633991331No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA9060.12109401627150723No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC8950.11962377987085979No Hit
ATTATATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC8800.11761891205179509No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG8710.11641599136035627No Hit
ATGTAAGAGTTAATAGTCTCAAACTGGCAGTCGGTCTGAGGAACGTGCCT8680.11601501779654334No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC8670.11588135994193903No Hit
CATTTATGGTATAGCTCGAAACAGCCTTTCCCATCTTCCATAGCATTGGA8640.11548038637812609No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA8400.1122725978676226No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT8390.11213894001301826No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT8320.1112033350307881No Hit
ATTTGGAGCCATAGCTGGATTCATAGAAGGAGGTTGGCCAGGACTAGTCG8260.1104013879031622No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC8170.10919846721172338No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT8140.10879749364791046No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA8060.10772823081107595No Hit
ATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAGACACA8000.10692628368345009No Hit
CTAATAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTG7910.10572336299201127No Hit
GTGCAATGTCAGACTGAAAAAGGTGGCTTAAACAGTACATTGCCATTCCA7770.1038521530275509No Hit
CCATTGGACATGGCCCAGAACAGGAAGGCAGCAAACCCCATTGCAAGCAC7710.10305020589992502No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA7670.10251557448150778No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC7650.10224825877229915No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA7610.10171362735388188No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC7500.10024339095323445No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC7490.10010973309863014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGC100.0070905364144.9998145
TGGGCGG301.10145265E-7120.83316145
GACTTAA256.463846E-6115.99984145
AGTAGAA7000.0114.987181
GCAGGTA3800.0110.6577452
GGTACTG4300.097.790565
GGTCAAA2500.087.017331
GCCTGCC259.3858707E-486.99988145
CAGGTAC4900.085.816213
AAGGTAG451.2325927E-680.555445
GTACTGA5250.080.095136
CGCAATT554.3930413E-879.090805145
AAATATA2750.079.09085
GCGAAAG1600.072.49992
ATACCAC502.305691E-672.4999145
AGAAACA11200.071.852584
TTTGGAC6450.070.813853
AGGTACT5950.070.672174
GACAGTA6500.070.2691357
TAGAAAC11850.067.91133
Produced by FastQC (version 0.11.5)