FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1987_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1987_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATGCCCAGTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACA30.20270270270270271No Hit
GGATTAGGTAGGAAGAGACTTGGAACATAGAAACTTAAAGAAAATCTTGG30.20270270270270271No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC20.13513513513513514No Hit
CCTTGGTGGTGTTTAGTCACTCAATGTTTATTCACTCGAGAGAAAACACT20.13513513513513514No Hit
AGGTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGAGAGGAATGGAGTCAG20.13513513513513514No Hit
CTTGATATCAAGTAGTGAAAATTTCAAGGAGAAAATAACGCCAAGCCCCT20.13513513513513514No Hit
TTTATACATATTCTCGTATTGGGAGTCAGGATATGGTCATTGGAGAAGAA20.13513513513513514No Hit
AGAGTAAGCCCTCAAAAAATGTTGTAAAAAATCTTATTCTGGGGGTACCT20.13513513513513514No Hit
TTCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATC20.13513513513513514No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT20.13513513513513514No Hit
GAAGTAGTCAGCAGCTCTTAGGGACCATTACACTCTTGGACTCAAATGAT20.13513513513513514No Hit
GTATTAAAGTATGGTATTGGTGCTGATTGCTTCTCTTAGGTTTGTTCATA20.13513513513513514No Hit
ATTTCAGTCCTCATTTGGGAAAATTCTAGGCTTCTGGAATTTATTGACTT20.13513513513513514No Hit
AATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCAG20.13513513513513514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC350.003324784820.714287130-134
Produced by FastQC (version 0.11.5)