FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1987_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1987_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGGGGAGAGGCAGGCAGAGGGAGAGGGAGAAGCAGGCTCTCCACA12.857142857142857No Hit
TTACTGTGTAGGAAGTAGTTGGTGCCCTAGTGACTCATATCTGCAGCGGG12.857142857142857No Hit
GTTATGGTCCATTTTGGTCTGAACACCTTTGTAAGCCCTACAGTTCCAGA12.857142857142857No Hit
CAACAGAGCGCTTGGTGTGGGGGAAATCCGTTCCAAACTGATGTCAGAAT12.857142857142857No Hit
CACCCTGTAGCTCGACCACGGGGAGTAGTGGCCGAAGCTGGGCTGAGGGG12.857142857142857No Hit
CCCAGGCGCCCCCAGAACCAACCGTTTTGATGCATCTCAGTGAACCAGTG12.857142857142857No Hit
GAACAGTCCTGATGCAGGAAAGACAGGGCCAATCCCAAGGATATGCAAGG12.857142857142857No Hit
GGAACCGGCTGCCACCGTGGCCTCTGTGGTGGCCACCTGCCCCGTGAGCT12.857142857142857No Hit
CACTCGCTTTCAGGTTTGTCCAGCTAGCCAAATGGAGGCAGAGGAGAGCC12.857142857142857No Hit
AACTGGATCAGTTGAGGCTCAGCAAGAACCAGATCTACAGAATCCACCCT12.857142857142857No Hit
CCCCCACACACAACCAAAGAATTATCTGGACTGAAATGCTGAAAAAACAC12.857142857142857No Hit
ATTGAAACCTCGCACAGGGTCCCCAGGGGCAGGCGGCACCTGGTGTTCTG12.857142857142857No Hit
AGCCAGGACCAGGCAGGGGGTAGAGCCCACGAGACAGGGACATCTAGAAT12.857142857142857No Hit
TCTAATGACAGTGCACACACTGCGCTCTTGATCCGCAGGGTCCTCAGTTC12.857142857142857No Hit
CTGGTTGCCACAAGTTGGTGAGGCTTGCTCGGTCATATAATGGAGACAGG12.857142857142857No Hit
GTCCATCCCCCTCTCCAGGTGTTGCCAGACCCACGACCACTGCTACTCAG12.857142857142857No Hit
TTTTAGTATTTAATGGTACCCGGGAGGAACCAGCACTTGGAACATGGGAA12.857142857142857No Hit
GTTAATTGCTAATAAAAATAATGGCCAGATGTTATTTGAGTGCCAATGGA12.857142857142857No Hit
GGCAATTTATTTTACATTACGTGGATATTACACAGACCGTGGCTGCTCTG12.857142857142857No Hit
GCTCCCCCTGCACAGAGGGACCCAATGGGATGCAGGGAAATTATACATGT12.857142857142857No Hit
GGTTTTCAGCTTGGCGCGAGCCGCACATCGCTGCTGCTTCCTCAGCAAGG12.857142857142857No Hit
GGCTTACTGGACACAAATATCTACTCTTGGAGAACTCTTCTTCCGCAGGT12.857142857142857No Hit
CAATATGATCAATAAGATGATTGATCACCAAATACAAGACCTATGCACAA12.857142857142857No Hit
CTTCTCCAGCCCTGCAACACAGCCACCACCTTCCCCTCCCAGGGCCTGGC12.857142857142857No Hit
CCTTATAAGAGAGTTGGGACTCTTACCTGTGCCAAGGAATACTTCACCAC12.857142857142857No Hit
GGCAGGAACCGCAGCCCGTAGAACCGCCTCTGATTTCCCAGTGAATGCTA12.857142857142857No Hit
ACCGCAGGGGGGCCAGCAGGCAGCGGCAGCAGGAGAAGCAGGCTCCCCCC12.857142857142857No Hit
GGAGAGAAGGGTGCAGCCCAAACCTGGTCGTCGGACAGCCTCCTCTTGTA12.857142857142857No Hit
CCACCGACTAATCGACTAGACTAATCGACTACTCGACGAGCCTAATCTAC12.857142857142857No Hit
CCGACATGCCAAATACGCGCCCAAGTGCGGGCGGGGACCATACCCGGGAA12.857142857142857No Hit
CCCATGGGCTGTGGGGCCAGAGGGCCTCAGAGCAGCTGACCCAGCCTGGA12.857142857142857No Hit
GATGGTTGTGATTACTAAGGCATACAATCAGATGCAAACGACTGCTTTCT12.857142857142857No Hit
GGCTGGCAGTAGAGACAGTGTGTCTATCAGGGTGGAGAATATTAGGACTG12.857142857142857No Hit
TCATAGACGGGTACCGAAGAAATCCCCAACCTGGACCCGCCAACTGGTAG12.857142857142857No Hit
CCTGATACGTGTAGAACCATGCACAGTATTTGTCCTTCGTTGACTGGCTT12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)