FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1987_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1987_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGT13.4482758620689653No Hit
GGACCTTTGCACTTGCTATTCTCTCTGCTGGCAACATTCAATTCCTAGCT13.4482758620689653No Hit
GTTTCATTACTGTTTCTGCTGCATCTTACACCGTAGCCAAGCCGCACCAC13.4482758620689653No Hit
CCCTAGTATTTCCCACCCACTAGGGTGCTTTCTGGGAGTAAAAGGAGATA13.4482758620689653No Hit
GTTCTATCCCCCGCTTCGTGATGTGCCTGGACCCATGGTATAGCGCCAGG13.4482758620689653No Hit
TGTGGAAGAGGAAGGGAGAGCCAACATCACGATGAAAAAGATAGAATATT13.4482758620689653No Hit
CCAGATAAATTCATAACCCCGTACTAAATGATCTCACAGGGGCTGCATTC13.4482758620689653No Hit
GTCTGGGGCAGTGCCAGTCCCTGGATGCGGACGGTGGGTCCCGAGCATTA13.4482758620689653No Hit
TCCTTAAAGATCACATATAATTATAGTTTCCCATAGAAACTCAATGCCGA13.4482758620689653No Hit
TATCTTACCAGCTATAGACAAAAAAAAACCATCCAAATCTGGATGGCTTT13.4482758620689653No Hit
GTACTGATATGTGCTACGATATAGATGAGCCTTGAAAACATCGTGTTAAG13.4482758620689653No Hit
TCGCAGTGATGCTTTGTTTGGGTGGTGTACTAGCTTCCTATAGCTGCTAT13.4482758620689653No Hit
GCAGGGGGGAGTGGCAGGCAGAGGGAGATGGAGAAGCAGACTCCCCACTG13.4482758620689653No Hit
GAGATAGAACCACATTTGCCAAGAGATCACACAAAAATAGTTTAAAATAG13.4482758620689653No Hit
CTTCTTTCTCTGGCCAGAAATATGAACTCTGATCCAAGGAAAGGAGAGCT13.4482758620689653No Hit
TATCGTGAGAGAGCTAACCCAGTCCAGCATGCGACAATGGGCATGCTCAG13.4482758620689653No Hit
GGCAGAGGGAGTGTGGAGCCCGACTTTGGGGCTCCCTCTACCAACCACAA13.4482758620689653No Hit
CATTTGGATGATTCTTCCAAATTTCTTCAGGCACAATTTTGCTCTTTGCT13.4482758620689653No Hit
TGGGAAAATGGAGGCTCAGAATGATTAAGCAGCTTGCCTTAAGTCACACA13.4482758620689653No Hit
GTCCAGGCTGCATATGAAGTGTTCTTAGGGGCTGAAGTTGAAGTACCTTC13.4482758620689653No Hit
CATCCGGCATTTATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTG13.4482758620689653No Hit
GCACAGGAGAAAAAAACGGGGCGATTCAGTGATGAGGTGTTGGAGGCATG13.4482758620689653No Hit
GCTGCGCAGACAAATATCTTCCAGCGCGTCCTGCGCACATGCGCCGTAAT13.4482758620689653No Hit
GTTGGGATCCAGACTGGTGAATATCCCGATCGGACAAAACCCACTAAATA13.4482758620689653No Hit
GGGCCTCACTGGCTGTCCCACAGAGACCTCCCTTCCTTGCCCCGTGGGCC13.4482758620689653No Hit
CAACTTCTTCCATCCCTCAAACACATACCATAATTCTAGTGTCAGATGCT13.4482758620689653No Hit
CCCCTTCCCCCACGCAGGTTCGCTTTTTAAAAGGGCTGACGGTCCCACCG13.4482758620689653No Hit
CTTTTAATGGCGGAGGCCGCCCTGGCCAGGGGCCTGCCACCGACACTTGG13.4482758620689653No Hit
CTATAAAAGCACCCAGAGCCACTCTACGAGTCCCCGGGCCATCCTTCAAC13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)